16 1 The Exploitation of Lipase Selectivities for the Production of Acylglycerols
Table 5.
Typical data for lipase-catalyzed monoglyceride synthesis.
Process
Lipase
T(^8
C)
Yield
1
(%)
Majorisomer
Product composition
2
(% w/w)
Reference
FFA
MG
DG
TG
Substrate: triglyceridesHydrolysis
Porcine pancreatic lipase
3
40
67
2
60
26
4
8
Plou et al., 1996
R. delemar
(microemulsion) 35
80
2
55
31
13
4
Holmberg and Osterberg, 1996
R. arrhizus
(solvent)
35
95
2
Millqvist et al., 1994
Alcoholysis
R. arrhizus
(solvent)
35
60–81 2
70
5
25
5
0
Millqvist Fureby et al., 1997Soumanou et al., 1998
Directedglycerolysis
Pseudomonas fluorescens
60–40 75–92 1(3)
1
22
75
2
McNeill and Berger, 1993
Pseudomonas cepacia
55–25 96
1(3)
n.a.
96
2
2
Bornscheuer et al., 1994
Chromobacterium viscosum
25–10 90
Rosu et al., 1997
Substrate: fatty acids/glycerolEsterification
Patatin
50
86
n.d.
6
89
5
0
Macrae et al., 1998
Penicilliumcamembertii
40
76
1(3)
20
72
8
0
Yamaguchi and Mase, 1991
P. cyclopium
(directed)
6
40
90
1(3)
10
90
0
0
Weiss, 1990
Rhizopus arrhizus
(directed) 20
80
1(3)
8
80
12
0
Weiss, 1990
1
Maximum yield on molar basis.
2
Product composition on weight basis in the lipid fraction, as recalculated from experimentaldata reported in literature.
3
Lipase immobilised on Celite.
4
Sum of diglycerides and triglycerides.
5
Sum of free fatty acids and ethylesters.
6
2–5% (w/w) water in glycerol.
