102 CATALYZING INQUIRY
FIGURE 4.4 Integration of protein family, pathway, and genome context data for disease gene identification.
The ATR enzyme (EC 2.5.1.17) converts inactive cobalamins to AdoCbl (A), a cofactor for enzymes in several
pathways, including diol/glycerol dehydratase (EC 4.2.1.28) (B) and methylmalonyl-CoA mutase (MCM) (EC
5.4.99.2) (C). Many prokaryotic ATRs are predicted to be required for EC 4.2.1.28 based on the genome context of
the corresponding genes. However, in at least one organism (Archaeoglobus fulgidus), the ATR gene is^ adjacent to
the MCM gene, which provided a clue for cloning the human and bovine ATRs.
SOURCE: Courtesy of Cathy Wu, Georgetown University.