22 A Practical Guide to Cancer Systems Biology
- (Optional) Transfer 1μL of the clear supernatant to a new PCR tube for
library validation.
IX.Validate library
- Load 1μL of the clear supernatant from the ligated DNA samples and the
PCR amplified DNA samples on an Agilent Technologies 2100 Bioanalyzer
using a DNA-specific chip. - Check the size and purity of the sample. The final product should be a
band at approximately 260 bp as shown in Fig. 3.
References
- Su Z, Li Z, Chen Tet al.(2011) Comparing next-generation sequencing and microarray
technologies in a toxicological study of the effects of aristolochic acid on rat kidneys.
Chem. Res. Toxicol.24:1486–1493. - Koboldt DC, Steinberg KM, Larson DEet al.(2013) The next-generation sequencing
revolution and its impact on genomics.Cell155:27–38. - Mardis ER. (2013) Next-generation sequencing platforms.Annu. Rev. Anal. Chem.6:
287–303. - Mutz KO, Heilkenbrinker A, L ̈onne Met al.(2013) Transcriptome analysis using next-
generation sequencing.Curr. Opin. Biotechnol.24:22–30. - Meldrum C, Doyle MA, and Tothill RW. (2011) Next-generation sequencing for cancer
diagnostics: A practical perspective.Clin. Biochem. Rev.32:177. - Rabbani B, Mahdieh N, Hosomichi Ket al.(2012) Next-generation sequencing: Impact
of exome sequencing in characterizing mendelian disorders.Clin. Biochem. Rev.57:
621–632. - Ekblom R and Galindo J. (2011) Applications of next generation sequencing in
molecular ecology of non-model organisms.Heredity107: 1 −15. - BerkmanPJ,LaiK,LorencMTet al.(2012) Next-generation sequencing applications
for wheat crop improvement.Am.J.Bot.99:365–371. - van Dijk EL, Auger H, Jaszczyszyn Yet al.(2014) Ten years of next-generation
sequencing technology.Trends Genet.30:418–426.