Species
Proteins
PRKAA1
PRKAA2
PRKAB1
PRKAB2
PRKAG1
PRKAG2
PRKAG3
1001_Chordata1002_Cnidaria
1003_Platyhelminthes
1004_Nematoda1005_Arthropoda
1006_Basidiomycota1007_Ascomycota1008_Opisthokonta1009_Amoebozoa
1010_Fornicata1011_Euglenozoa1012_Apicomplexa
1013_Bacillariophyta1014_Streptophyta1015_Parabasalia1016_Euryarchaeota1017_Crenarchaeota
1018_Candidatus Korarchaeota
1019_Proteobacteria1020_Fusobacteria1021_Nitrospirae1022_Dictyoglomi
1023_Aquificae
1024_Thermotogae1025_Spirochaetes1026_Planctomycetes1027_Chlamydiae1028_Bacteroidetes1029_Cyanobacteria1030_Firmicutes
1031_Deinococcus−Thermus
1032_Chloroflexi1033_Actinobacteria
0.250.50
0.751.00
FAS
presSpec0.00
0.250.50
0.751.00
0
Traceability
(A) (B)
(C)
0
0.97
0 0 0
0.03
cast_S−rich
pfam_AdenylateSensor
seg_lowcomplexityregions
smart_RIO
PRKAA1:HUMAN - 0.976
PRKAA1:DROME - 0.976
0.84
0 000
0.03
0
0.01
0
0
0.09
0.01
0
0
pfam_AdenylateSensor
seg_lowcomplexityregions
smart_RIO
cast_P−rich
pfam_Kinase−like
pfam_Pkinase
pfam_Pkinase_Tyr
pfam_RIO1
smart_KIND
smart_S_TKc
smart_TyrKc
0 200 400 600
(^0200400600)
Fig. 7Visualization of phylogenetic profiles using PhyloProfile. (a) Phylogenetic profile of the human AMPK
pathway. The color code represents the FAS score. (b) Customized phylogenetic profile for seven AMPK
proteins: PRKAA1, PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, and PRKAG3. Species are summarized on
the phylum level. The size of the dots indicates the fraction of species in a phylum with an ortholog present. (c)
Domain architecture comparison between human PRKAA1 (above) and its ortholog in yeast (below). Note that
the FAS-S algorithm ignores the accumulation of redundant features annotated in the N-terminal half of the
yeast ortholog. All main features of the human protein are observed also in the yeast sequence, and hence we
have no reason to assume functional divergence of the two proteins
Tracing AMPK Evolution 129