PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PK
PH PK PKC
C1 C1C1C1 C2C1C 1 PK PKC
C1 C1C1C1 C2C1C^1 PK PKC
C1 C1C1C1 C2C1C 1 PK PKC
C1 C1C1C1 C2C1C 1 PK PKC
C1 C1C1C1 PK PKC
PH PK PKC
PH PK PKC
PH PK PKC
PK PKC
PK PKC
PK PKC
PK PKC
PH PK PKC
PK PKC PK
PK
DUF3543
DUF3543
MIT
MIT
MIT
MIT
MIT
MIT
DUF3543
DUF3543
DUF3543
PK
PKCPKC
DUF3543
PH PKCPKC
PK
PK PK
PK
DUF3543
Human RPS6K A PK PKC
Human RPS6K B
Fungi RPS6K B - like
Plants RPS6K B - like
Nemve RPS6K A
Nemve RPS6K B
Human AKT 1
Human AKT 2
Human AKT 3
Drome AKT 1/2/3
Human PRKC A
Human PRKC B
Human PRKC G
Drome PRKC
Fungi PRKC
Dicdy AKT-like
Human CAMKK 1
Human CAMKK 2
Drome CAMKK
Fungi CAMKK
Plants CAMKK
Human STK 11
Nemve STK 11
Fungi STK 11
Plants
Plants
Human PRKAA 1
Human PRKAA 2
Drome PRKAA
Fungi PRKAA
Plants PRKAA
Human MAPK 1
Human MAPK 3
Drome MAPK
Fungi MAPK
Plants MAPK
KA1
Human ULK 1
Human ULK 2
Drome ULK 1/2
Nemve ULK 1/2
Human ULK 3
Drome ULK 3
Nemve ULK 3
Fungi ULK 1/2 - like
Plants ULK 1/2 - like
LECA
1 2 3 4 5 6 7
1 2
3 - 6 7
gene loss
gene loss
Fig. 12A hypothetical evolutionary scenario of eukaryotic kinase evolution. The figure integrates the results
from phylogenetic tree reconstruction and Pfam domain architecture analysis. The phylogenetic relationships
of contemporary kinases imply the existence of seven proto-kinases in the last eukaryotic common ancestor
(LECA). A parsimony criterion was used to infer the Pfam domain architecture of these ancestral proteins
(numbers 1–7 below the tree).See[12] for details
Tracing AMPK Evolution 137