AMPK Methods and Protocols

(Rick Simeone) #1

  1. Viollet B, Andreelli F, Jorgensen SB, Perrin C,
    Geloen A, Flamez D, Mu J, Lenzner C,
    Baud O, Bennoun M, Gomas E, Nicolas G,
    Wojtaszewski JF, Kahn A, Carling D, Schuit
    FC, Birnbaum MJ, Richter EA, Burcelin R,
    Vaulont S (2003) The AMP-activated protein
    kinase alpha2 catalytic subunit controls whole-
    body insulin sensitivity. J Clin Invest 111
    (1):91–98. https://doi.org/10.1172/
    JCI16567

  2. O’Neill HM, Maarbjerg SJ, Crane JD,
    Jeppesen J, Jorgensen SB, Schertzer JD,
    Shyroka O, Kiens B, van Denderen BJ, Tarno-
    polsky MA, Kemp BE, Richter EA, Steinberg
    GR (2011) AMP-activated protein kinase
    (AMPK) beta1beta2 muscle null mice reveal
    an essential role for AMPK in maintaining
    mitochondrial content and glucose uptake dur-
    ing exercise. Proc Natl Acad Sci U S A 108
    (38):16092–16097. https://doi.org/10.
    1073/pnas.1105062108

  3. Viollet B, Athea Y, Mounier R, Guigas B,
    Zarrinpashneh E, Horman S, Lantier L,
    Hebrard S, Devin-Leclerc J, Beauloye C,
    Foretz M, Andreelli F, Ventura-Clapier R, Ber-
    trand L (2009) AMPK: lessons from transgenic
    and knockout animals. Front Biosci (Landmark
    Ed) 14:19–44

  4. Viollet B, Foretz M (2016) Animal models to
    study AMPK. EXS 107:441–469.https://doi.
    org/10.1007/978-3-319-43589-3_18

  5. Foretz M, Hebrard S, Leclerc J,
    Zarrinpashneh E, Soty M, Mithieux G,
    Sakamoto K, Andreelli F, Viollet B (2010)
    Metformin inhibits hepatic gluconeogenesis in
    mice independently of the LKB1/AMPK path-
    way via a decrease in hepatic energy state. J Clin
    Invest 120(7):2355–2369. https://doi.org/
    10.1172/JCI40671

  6. Cao J, Meng S, Chang E, Beckwith-Fickas K,
    Xiong L, Cole RN, Radovick S, Wondisford
    FE, He L (2014) Low concentrations of met-
    formin suppress glucose production in hepato-
    cytes through AMP-activated protein kinase
    (AMPK). J Biol Chem 289
    (30):20435–20446. https://doi.org/10.
    1074/jbc.M114.567271

  7. Fullerton MD, Galic S, Marcinko K, Sikkema S,
    Pulinilkunnil T, Chen ZP, O’Neill HM, Ford
    RJ, Palanivel R, O’Brien M, Hardie DG,
    Macaulay SL, Schertzer JD, Dyck JR, van Den-
    deren BJ, Kemp BE, Steinberg GR (2013) Sin-
    gle phosphorylation sites in Acc1 and Acc2
    regulate lipid homeostasis and the insulin-
    sensitizing effects of metformin. Nat Med 19
    (12):1649–1654. https://doi.org/10.1038/
    nm.3372

  8. Zhou G, Myers R, Li Y, Chen Y, Shen X,
    Fenyk-Melody J, Wu M, Ventre J, Doebber T,


Fujii N, Musi N, Hirshman MF, Goodyear LJ,
Moller DE (2001) Role of AMP-activated pro-
tein kinase in mechanism of metformin action.
J Clin Invest 108(8):1167–1174.https://doi.
org/10.1172/JCI13505


  1. Yu PB, Hong CC, Sachidanandan C, Babitt JL,
    Deng DY, Hoyng SA, Lin HY, Bloch KD,
    Peterson RT (2008) Dorsomorphin inhibits
    BMP signals required for embryogenesis and
    iron metabolism. Nat Chem Biol 4(1):33–41.
    https://doi.org/10.1038/nchembio.2007.54

  2. Hao J, Daleo MA, Murphy CK, Yu PB, Ho JN,
    Hu J, Peterson RT, Hatzopoulos AK, Hong
    CC (2008) Dorsomorphin, a selective small
    molecule inhibitor of BMP signaling, promotes
    cardiomyogenesis in embryonic stem cells.
    PLoS One 3(8):e2904.https://doi.org/10.
    1371/journal.pone.0002904

  3. Yu PB, Deng DY, Lai CS, Hong CC, Cuny
    GD, Bouxsein ML, Hong DW, McManus
    PM, Katagiri T, Sachidanandan C, Kamiya N,
    Fukuda T, Mishina Y, Peterson RT, Bloch KD
    (2008) BMP type I receptor inhibition reduces
    heterotopic [corrected] ossification. Nat Med
    14(12):1363–1369. https://doi.org/10.
    1038/nm.1888

  4. Shen Q, Little SC, Xu M, Haupt J, Ast C,
    Katagiri T, Mundlos S, Seemann P, Kaplan FS,
    Mullins MC, Shore EM (2009) The fibrodys-
    plasia ossificans progressiva R206H ACVR1
    mutation activates BMP-independent chon-
    drogenesis and zebrafish embryo ventraliza-
    tion. J Clin Invest 119(11):3462–3472.
    https://doi.org/10.1172/JCI37412

  5. Kaplan FS, Xu M, Seemann P, Connor JM,
    Glaser DL, Carroll L, Delai P, Fastnacht-
    Urban E, Forman SJ, Gillessen-Kaesbach G,
    Hoover-Fong J, Koster B, Pauli RM,
    Reardon W, Zaidi SA, Zasloff M, Morhart R,
    Mundlos S, Groppe J, Shore EM (2009) Clas-
    sic and atypical fibrodysplasia ossificans pro-
    gressiva (FOP) phenotypes are caused by
    mutations in the bone morphogenetic protein
    (BMP) type I receptor ACVR1. Hum Mutat 30
    (3):379–390. https://doi.org/10.1002/
    humu.20868

  6. Shore EM, Xu M, Feldman GJ, Fenstermacher
    DA, Cho TJ, Choi IH, Connor JM, Delai P,
    Glaser DL, LeMerrer M, Morhart R, Rogers JG,
    Smith R, Triffitt JT, Urtizberea JA, Zasloff M,
    Brown MA, Kaplan FS (2006) A recurrent
    mutation in the BMP type I receptor ACVR1
    causes inherited and sporadic fibrodysplasia ossi-
    ficans progressiva. Nat Genet 38(5):525–527.
    https://doi.org/10.1038/ng1783

  7. Hao J, Ho JN, Lewis JA, Karim KA, Daniels
    RN, Gentry PR, Hopkins CR, Lindsley CW,
    Hong CC (2010) In vivo structure-activity
    relationship study of dorsomorphin analogues


Compound C: Its Use and Misuse 201
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