- This step is required to allow cardiomyocytes to recover their
basal state before to start hypertrophic treatment. - 100 nM angiotensin II can be equally used to induce neonatal
cardiomyocyte hypertrophy [21]. - Protein synthesis (radioactiveL-phenylalanine incorporation)
can be followed during shorter incubation time. Indeed, this
Fig. 4AMPK activation inhibits phenylephrine-mediated increase in ERK1/2 phosphorylation and NFAT
transcriptional activity. (a) NRVMs were treated with (open bars) or without (solid bars) phenylephrine
(PE) in the presence or absence of phenformin (1 mM) for 24 h. Evaluation of NFAT transcriptional activity
by luciferase activity measurement.N¼4. (b,c) NRVMs were treated with (open bars) or without (solid bars)
phenylephrine (PE) in the presence or absence of phenformin (1 mM) for 1 h. Representative immunoblot and
quantification of ERK1/2 (Thr202/Tyr204) phosphorylation state and ERK1/2 total content.N¼3. Data are
means.e.m. and were analyzed using two-way ANOVA followed by Bonferroni post test (*p<0.05
vs. untreated cells,#p<0.05 vs. PE-treated cells)
Study of Cardiac Hypertrophy and Protein Synthesis 339