GLUT4 translocation in cardiomyocytes upon long-term
(18 h) treatment, indicating reliance on synthesis of specific
proteins. These proteins might be involved in the negative
regulation of GLUT4 endocytosis [39].Fig. 2Effects of Compound C on substrate uptake into rat cardiomyocytes stimulated by various AMPK
activators. Cell suspensions were incubated in absence or presence of 50μM Compound C for 30 min, and
subsequently for another 15 min without or with 5 μM oligomycin or 1.5 mM AICAR. Alternatively,
electrostimulation was performed for 7 min at 4 Hz. Then, radioactive substrates were added for
measurement of (a) palmitate uptake or (b) deoxyglucose uptake during 5 min. Data are meansS.E.M
(n¼4–6).*Significantly different from myocytes without additions (Ctrl) (p<0.05).^Significantly different
from corresponding incubations in the absence of Compound C (Basal) (p<0.05)
354 Joost J. F. P. Luiken et al.