- Phosphate-buffered saline (PBS): 145 mM NaCl, 2.7 mM KCl,
1.5 mM KH 2 PO 4 , 8 mM Na 2 HPO 4 ,pH7. - Flow medium: PBS, 0.1% (w/v) BSA, 0.1% (w/v) NaN 3.
Fig. 2Combination of bead-based cell counting and flow cytometry analysis allows calculation of T cell
recovery upon metabolic stress. Splenocytes from AMPKα 1 flox/flox-CD4-CRE+mice were incubated for 6 h with
(a) DMSO or (b) 10 nM oligomycin. Splenocytes from left panels were gated for single cells only (exclusion of
doublets), then gated for viability (exclusion of dead cells), and further gated for B cells or T cells, as indicated
in right panels. Percentages or numbers of cells in each gate are indicated. (c) Calculation of relative viable T
cell recovery from examples in (a,b)
468 Se ́bastien Denanglaire et al.