Fig. 1AMPK expression constructs in bacteria and insect cells. (a) Diagram of recombinant human AMPK
heterotrimeric construct inserted betweenNcoIandXhoIsites of pET14bE. coliexpression vector. (b)
Schematic representation of heterotrimer expression strategy in Sf-9 eukaryotic cells
36 Ravi G. Kurumbail et al.