AMPK Methods and Protocols

INDEX

A

A-769662..................................4,15–17, 22, 24, 25, 70,
295, 355, 373, 375, 379, 380, 382, 386, 387,
480–484, 488, 490, 491
Acetyl CoA carboxylase (ACC)..........................108, 192,
215, 242, 249, 266, 363, 367, 368, 373, 377,
380, 382, 386, 388, 393, 395, 399, 404, 415,
417, 422–424, 428
Activation
allosteric...................................................1, 15, 16, 18,
31, 45, 51, 69–71, 73, 74, 76, 77, 79, 80, 83,
215, 216, 229, 241, 249, 250, 295, 481, 594
Activator..............................................229, 239, 250, 368
dominant-positive mutant....................241, 583, 608
small molecule/pharmacological
ADaM activator..... 4, 13, 18, 20, 44, 74, 82, 250
indirect............................................................... 368
nucleotide-binding site activator.............229, 239
Activity,seeKinase activity
Acute myeloid leukaemia (AML)..... 172, 174, 181, 182,
186–188, 192
Adenine nucleotides
ADP ......................................230–232, 241, 251, 395
AMP.............................230–232, 241, 251, 395, 597
ATP ..............................230–232, 241, 251, 395, 597
quantification...........................................................230
Adenovirus..................................................161, 309, 335,
341, 406, 421, 427, 440, 444, 599
Allosteric,seeActivation
AMARA peptide................................................73, 77, 79,
216, 218, 226
5-Aminoimidazole-4-carboxamide ribonucleotide
(AICAr).................................................. 42, 43,
199, 240, 246, 295, 309, 323, 324, 326, 327,
329, 332, 335, 337, 352–354, 356, 357, 373,
375, 379, 380, 382, 386, 387, 414, 422, 439,
453, 454, 457, 459, 478, 481, 541, 545, 552,
553, 558, 561
AMP-activated protein kinase (AMPK)
AMPKα1........................................................161, 414,
437, 440, 445, 466, 473, 478
AMPKα2......................161, 176, 357, 437, 445, 509
AMPKβ1..................................................................161
AMPKβ2..................................................................161
AMPKγ1 ..................................................................208

AMPKγ2 .................................................208, 581–611

AMPKγ3 ..................................................................220

Amplex red ...................................60,496, 497, 500, 501

Angiogenesis........................................................197, 258,

519, 520, 522–529, 531–536

Animal models.....................................................460, 588,

594, 596, 597, 604

Caenorhabditis elegans.............................59,566, 567

mouse (Mus musculus)

transgenic........................588, 594, 596, 597, 604

knock-out.......................171–193, 195, 355, 374

rat (Rattus rattus)...................................................460

Antibody

isoform-specificity..................................................... 70

specificity .......................................203–205, 215, 216

Aorta

isolation...................................................................511

Atherosclerosis....................................307, 477, 478, 508

Autophagy

flux analysis....................................374, 379, 381, 382

AXIN.....................................................................393–409

B

Bafilomycin A.............................374, 375, 381, 382, 387

Bait plasmid.......................144, 145, 150–152, 154, 155

Baculovirus ...................................................................... 29

β-galactosidase......................................................327, 335

Biochemical titration....................................................... 59

Biochemistry.........................................................451, 532

Bioenergetic profile..............................................552, 559

Bioenergetics.....................273–282, 284, 285, 552, 559

Bioinformatics.......................................................99–103,

105–108, 111, 112, 173, 188

Biosensor..................256, 258, 259, 261, 265, 270, 271

C

Ca2+/calmodulin-activated protein

kinase 2 (CaMKK2).........................17, 19, 22,

25, 69, 72, 75–77, 81, 83, 160, 162, 165, 166,

169, 249, 433

Cancer

metabolism......................... v, 15,159, 172, 196, 307

Capillary electrophoresis (CE) ............................. 74,175,

184, 186, 187, 192, 241, 243, 246, 247, 395,

398, 404, 406, 478

Dietbert Neumann and Benoit Viollet (eds.),AMPK: Methods and Protocols, Methods in Molecular Biology, vol. 1732,
https://doi.org/10.1007/978-1-4939-7598-3,©Springer Science+Business Media, LLC 2018

621