AMPK Methods and Protocols

L

Lactate........................................257, 285, 290, 301, 303
LC3,seeMicrotubule-associated protein 1A/1B-light
chain 3 (LC3)
Left ventricular hypertrophy (LVH)...........................581,
582, 584, 585, 587, 588, 593, 594, 596, 598,
600, 605–609
Leishmaniasis........................................................551–562
Lentivirus.............................................................174, 176,
180, 181, 184, 187, 188, 191
Ligand
binding........................................................... 2,29–31,
42, 48, 73, 79, 80, 87, 89, 97
Lipid...............................................................................477
extraction................................................364–366, 369
non saponifiable.............................................365, 369
saponifiable............................................364, 366, 368
synthesis..........................................................363–369
uptake,seeLong-chain fatty acid uptake
Lipogenesis....................................................................364
Liquid chromatography–mass spectrometry (LC-MS) 38,
241, 243, 244, 246, 247
Liver kinase B1 (LKB1).......................................... 69, 74,
76, 81–84, 160, 198, 243, 249, 255, 260, 264,
348, 357, 393–399, 401–409, 414, 433, 552,
558, 561, 597
Localization
lysosomal.......................................373, 396, 399, 402
nuclear...................................................258, 259, 399
Long-chain fatty acid (LCFA)
uptake.............................................................343–358
Lymphocyte
identification...................................................466, 474
purification ..............................................................466
survival.....................................................................465
Lysate
bacterial....................................................................100
cell...............................22, 70, 73, 79,100, 164, 165,
181, 234, 245, 249, 335, 387, 421, 514
kidney..............................................................450, 460
tissue....................................... 73,215, 232, 234, 514
Lysosome 257, 258, 268, 373, 374, 379, 382, 393–396,
399, 401, 402, 407–409

M

Macrophage
isolation.................................................479, 480, 557
Mammalian cell,seeCells
Matrigel plug assay..............................521, 523, 524, 528
Metabolism..........................................................1, 58, 59,
172, 196, 240, 289, 290, 298, 301, 303, 307,
321, 343, 346, 353, 357, 363, 465, 466, 478,

491.See alsoCancer metabolism;

Cell metabolism

Microtubule-associated protein 1A/1B-light chain

3 (LC3)........................................................374

Mitochondria

permeabilization....................................274, 281, 282

poison......................................................................465

respiration......................................................275, 279,

280, 284, 285, 290–293, 295, 297–300, 303

superoxide...............................................................514

Monocyte

adhesion.................................................307, 308, 315

Muscle..................................................................116, 121,

131, 139, 196, 197, 203–209, 211, 212, 216,

218, 225, 242, 257, 343, 348, 351, 353,

355–357, 539, 540, 542, 544, 548.See also

Skeletal muscle

Mutation

genetically engineered mutant ...............................450

PRKAG2 gene................................................581–612

Myogenesis..................................................539, 540, 547

Myogenic differentiation (MyoD).....................539, 540,

546–548

N

Neurons

culture....................................................290, 295, 299

metabolism..............................................................290

Nitric oxide (NO) ...............................................495–497,

499, 500, 532

Nuclear factor-κB (NFκB)..................................307, 309,

311, 313, 317

Nuclear factor of activated T-cells (NFAT)................322,

323, 327, 328, 335, 337, 339

Nuclear localisation.......................................................309

Nuclear magnetic resonance (NMR)..............88–97, 599

Nucleotide-binding...............................16,229, 583, 605

O

Organelles

light.......................................394, 395, 401, 404, 405

Ortholog

targeted search........................................................122

Oxidative phosphorylation (OXPHOS).....................273,

274, 279, 282, 353

Oxiprobe.......................................................................... 62

Oxygraph.............................................278, 279, 281–284

P

Pax7 ............................................539, 540, 542, 545, 546

Peroxynitrite.........................................................496, 504

Pharmacological activator,seeActivator

624
AMPK:METHODSANDPROTOCOLS
Index