Esophageal Adenocarcinoma Methods and Protocols

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4 Notes

1. Advantages and Disadvantages of TMA
   The advantages of using TMA are:
   ●● Reduction of coast in reagents, labor-effectiveness, and
   time of processing.
   ●● Preservation of the “donor” paraffin block for further
   analysis. This is especially important as more and more
   markers have to be tested in the cancer tissue.
   ●● Utilization of small quantities of tissue for the studies
   (minimizing the use of reagents). In a large section, a vari-
   ety of tissue, such as smooth muscle, normal epithelium,

Fig. 3 The finished TMA block (by manual method). (a) Showing the depth of tissue in the TMA block. (b)
Showing the cross section of (a) and labeled the sites of the tested samples and controls

Fig. 4 Sections taken from TMA block. (a) Hematoxylin and eosin-stained section cut from the TMA block of
esophageal adenocarcinoma. (b) Higher magnification of (a) showing the control tissue (normal lung) and the
cancer tissues from patients (three samples from patient 6 and three samples from patient 7)

TMA and Esophageal Adenocarcinoma