Esophageal Adenocarcinoma Methods and Protocols

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3 Methods

●● Turn on the power of scanner and then switch on computer

monitor.

●● When the status screen indicates “scanner ready,” the scanner

is ready for use.

●● Login to system with password.

●● Open the scanner door; take out the autoloader slide rack.

●● Load the slides to autoloader slide racks. Make sure the slides

are in the right position (i.e., glass coverslip face up and slide

label up to the front). After loading the slides to the rack, put

back the slide rack in scanner and close the scanner door.

●● Avoid slides with excessive labeling as it may cause jamming in

the slide rack.

●● The program will show up the images of the slide rack.

●● Select the racks with the slides inside for scanning (Fig. 2 ).

●● Make snapshots for these slides using autofocusing—this will

select the areas with the tissue.

●● Review the snapshots captured (Fig. 3 ).

●● Design the setting: select the designed scan magnification (5×,

10×, 20×, or 40×).

●● Data entry step: specify and save a name for each slide in the

name field.

●● Select the slide type and setting (see Note 3).

3.1 System Start-up

3.2 Loading
the Slides
for Scanning (Fig. 1 )
(See Note 2)

3.3 Set
up the Scanning Field

Fig. 1 Slide rack with multiple slides loaded up for scanning

Whole-Slide Imaging in Esophageal Adenocarcinoma