1414 Notes
- If the glass cover is too thick, use manual focusing and new
focus points are selected which are relatively closer to the tis-
sue. In addition, if there are slides with air pockets (bubbles)
under glass coverslip, perform a manual scan and avoid select-
ing the area of interest with air pockets. - If the slide is sitting on a non-flat position after lodging or
rocking while scanning, individual strips of scanned image will
be poorly focused. If this happens, the slides should be reposi-
tioned in the slide rack and scanned again. - Different types of sections need different setup for better scan-
ning. The typical setup is for a standard hematoxylin- and
eosin-stained section. However, the setting (slide type) could
be different for tissue section such as tissue microarray, serial
section, cytology, blood smear, very faintly stained section, and
in situ hybridization. To save time, we can preset and save
some of the chosen settings (slide setting) suitable for use in
our research group, and use the same setting next time. - The preselected area in the slide for scanning need to be adjusted
manually because there are some portions of the slides that the
operator may not want to scan. This will reduce the area needed
for scanning and hence increase the speed of scanning. - Although the scanning is automatic, manual work is required
for setup (Subheadings 3.1–3.3). The manual part of the works
depends on the operator’s experience. Time is needed for the
manual steps as well as for the scanning steps. Do not believe
in the time requirement provided by the individual company as
they will provide the shortest time required. The most com-
mon setting for scanning of hematoxylin- and eosin-stained
sections is at 40× magnification. By experience, we have calcu-
lated that the average time for scanning of slides in this setting
was approximately 12 min per slide.
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