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function of Trp53, but not Apc, leads to the - Follow steps 1– 3 in Subheading 3.2 to expose the distal
esophagus. - Carefully lift the stomach to expose its dorsal side.
- Ligate the distal esophagus with a 5-0 silk ligature and cut at
the esophagogastric junction. - Ligate the duodenum at the pyloric ring and remove the whole
stomach. - After confirmation of hemostasis, close the abdominal wall and
the skin separately with 6-0 Vicryl.
4 Notes
- Use an operating microscope (25×).
- Maintain the body temperature intraoperatively by using a
heating pad. - Before closing abdomen, fill the peritoneum with saline solu-
tion to replace intraoperative volume loss [ 10 ]. - Administer analgesic to alleviate pain on postoperative day 1
(buprenorphine 0.05 mg/kg, SC). - Deprive mice of food for 24–48 h postoperatively, but allow
free access to drinking water. - Assess the general health condition of the mouse on a daily
basis after surgery. If the mouse becomes hyposthenic, eutha-
nize the mouse.
References
3.4 Esophagogastro-
duodenal Anastomosis
with Gastrectomy: DER
(Fig. 3 ) [ 18 , 21 ]
Reflux Mouse Models in Esophageal Adenocarcinoma