157- Perform mice survival study [ 12 , 29 ] with paclitaxel and car-
boplatin treatments (Fig. 3 ) [ 12 ]. Inject 10 × 106 esophageal
adenocarcinoma OE19 cells intraperitoneally in each 4–6-week-
old female SCID mouse. Randomly group (n = 5 per group)
mice 2 weeks after OE19 cells injection. Treat mice intraperi-
toneally with vehicle, paclitaxel (20 mg/kg, two times a week
for 2 weeks) or carboplatin (50 mg/kg, two times a week for
2 weeks). Evaluate mouse survival from the first day of treat-
ment until death (see Note 33). - Euthanize mice by CO 2 exposure followed by cervical disloca-
tion according to IACUC-approved procedures.
Fig. 1 Formation of peritoneal tumor nodules and bloody ascites in SCID mice after intraperitoneal injection of
5 × 106 cells. (a) No peritoneal tumor formation was observed even 4 months (120 days) after intraperitoneal
injection of OACM5.1C cells. (b) Multiple peritoneal tumor formation with bloody ascites was observed
~55 days after intraperitoneal injection of OE19 cells. (c) Multiple peritoneal tumor formation but no bloody
ascites was observed in OE33 cells ~65 days after intraperitoneal injection. (d) Similarly, in SK-GT-2 cells
multiple peritoneal tumor formation without bloody ascites was observed ~76 days after intraperitoneal injec-
tion. Blue arrows show the tumors (published in PLoS One. 2017;12(6):e0180146 and permission obtained
from publisher to reuse)
Fig. 2 Ascites in SCID mice after intraperitoneal injection of 5 × 106 cells. (a, b) There was no ascites observed
in OACM5.1C cells. (c, d) Ascites was observed in OE19 cells (published in PLoS One. 2017;12(6):e0180146
and permission obtained from publisher to reuse)
Xenograft Models of Esophageal Adenocarcinoma