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Surg 144:1192–1199 - Keep the samples cold and protect from light exposure during
analysis for further functional studies using the sorted cells. - Seed the esophageal adenocarcinoma cells at different concen-
trations and in 12-well plates at each concentration. These can
give more reliable and comprehensive results of the spheres for
the SP-positive cells isolated from fluorescence- activated cell
sorting analysis. Do not change the media and disturb the
spheres formation during the incubation period. - Cells can be damaged with small gauge needle like 27- or
30-gauge needles. Use 22-gauge needles if significant number
of cells damaged with small needles. Place the syringe contain-
ing cells on ice to prevent further damage of the cells.
References
Farhadul Islam et al.