Esophageal Adenocarcinoma Methods and Protocols

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Ion AmpliSeq™ Adapters.
Platinum™ PCR Super Mix High Fidelity.
Library Amplification Primer Mix.
Low TE.
Barcode Adapters Kit (Each kit provides 16 different barcode
adapters. These barcode adapters are required to run multiple
libraries per sequencing chip).
Ion OneTouch™ 2 Instrument.
Ion PGM™ Hi-Q™ OT2 kit.
Ion PGM™ Sequencing Supplies which contain:
Wash 1 Bottle w/ label (250 mL).
Wash 3 Bottle w/ label (250 mL).
Ion PGM™ Reagent Bottle Sipper Tubes (blue).
Ion PGM™ Wash Bottle Sipper Tubes (gray; for 250-mL
bottles).
Ion PGM™ Wash Bottle Sipper Tubes (gray, for 2-L
bottles).
Reagent Bottles with labels (50 mL).
Ion PGM™ Hi-Q ™ Sequencing Reagents that contain:
Ion PGM™ Hi-Q™ Sequencing Polymerase.
Sequencing Primer.
Control Ion Sphere™ Particles.
Ion PGM™ Hi-Q™ Sequencing Solutions that contain:
Ion PGM™ Hi-Q™ Sequencing W2 Solution.
Ion Cleaning Tablet 4 tablets.
Annealing Buffer 12 mL.
Ion PGM™ Sequencing W3 Solution.
Ion PGM™ Hi-Q™ Sequencing dNTPs that contain:
Ion PGM™ Hi-Q™ Sequencing dGTP (black cap).
Ion PGM™ Hi-Q™ Sequencing dCTP (blue cap).
Ion PGM™ Hi-Q™ Sequencing dATP (green cap).
Ion PGM™ Hi-Q™ Sequencing dTTP (red cap).
The Ion PGM™ Wash 2 Bottle Kit.
Ion 316™ Chip v2 BC.
Ion PGM™ System and accessories.
Ion PGM™ Torrent Server.

Next Generation Sequencing and Esophageal Adenocarcinoma