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10. As multiple samples libraries will be loaded for each sequenc-
    ing chip, each sample must be assigned a unique barcode to
    enable sample distinction.


11. For each barcode X chosen, a separate mix of Ion P1 Adapter
    and Ion Xpress™ Barcode X was made. This consisted of: 2 μL
    Adapter P1, 2 μL Ion Xpress Barcode, and 4 μL nuclease-free
    water.


12. Diluted adapters could be stored at − 20 °C for multiple uses.
    The designated barcode number was recorded on the sample
    tracking sheet.

Fig. 1 Thermal cycler for amplification of target genomic DNA regions. The device

has thermal block with holes (arrow) where tubes holding the reaction mixture

can be inserted. The cycler then raises and lowers the temperature of the block

in preprogrammed steps

Table 3

Thermal program for digestion of amplicons

Temperature (°C) Time

50 10 min

55 10 min

60 20 min

10 Hold (for up to 1 h)

Suja Pillai et al.