Esophageal Adenocarcinoma Methods and Protocols

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2 Materials



  1. Total RNA extraction kit.

  2. Chloroform.

  3. 1.5 μL tube.


2.1 Isolation of Total
RNA


Fig. 1 Schematic presentation of miRNAs detection and quantification in esophageal adenocarcinoma. Total RNA
including miRNAs was extracted from the samples obtained from patients with esophageal adenocarcinoma and
controls. For analysis of global miRNAs expression using microarrays, these samples were labeled and hybridized.
Then the microarray plates were scanned and then the data were analyzed and presented. For detection and
quantification of target miRNAs in esophageal adenocarcinoma and control samples by RT-qPCR, cDNA was
generated from extracted RNA using reverse transcriptase reaction. After that qPCR reaction was carried out
using SYBR green or TagMan Probes system and finally data were normalized, analyzed, and presented


MicroRNA Detection and Esophageal Adenocarcinoma
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