Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

Reagents

Blank S

1

S^2

S^3

S^4

S^5

T^1 (fasting)

T^2 (30 min)

T^3 (60 min)

T

4

(90 min)

T^5 (120 min)

T^2 (150 min)

Phosphate buffer(ml)

1.9

1.8 1.8 1.8 1.8 1.8 1.8

1.8

1.8

1.8

1.8

1.8

Working reagent(ml)

1 1 11 11 1

1

1

1

1

1

Glucose standard(ml)

0.1 0.1 0.1 0.1 0.1

––––––

Glucose amount(mg)

0.05 0.1 0.15 0.2 0.25

Serum/plasma (ml)

––––––

0.1

0.1

0.1

0.1

0.1

0.1

Mix well and keep at 37

C for 5 min and read at 520 nm

15.5 Procedure 65