Table 8.1
Developed RNAi switches.
References
An
et al
. [10]
Beisel
et al
. [11]
Kumar et al.
[12]
Masu et al
. [13]
Beisel
et al
. [14]
Kumar
et al
. [15]
Saito
et al
. [16]
Kashida et al
. [17]
Afonin et al
. [18]
Velagapudi et al
. [19]
Input
Small molecule (theophylline)
Small molecule (theophylline, hypoxanthine)
Small molecule (theophylline)
RNA
Small molecule (theophylline, hypoxanthine)
Chemically modified RNA
Archaeal protein (L7Ae)
Human protein (U1A, NF
κB)
Chemically modified DNA
Small molecule library
Dose
~10
mM
~5
mM
~3
mM
~500
nM
~5
mM
~500
nM
Plasmid expression
Plasmid expression
~500
nM
~40
μM
Based RNA
shRNA
shRNA
Pri‐miRNA
siRNA
Pri‐miRNA
Pri‐miRNA
+
siRNA
shRNA
shRNA
siRNA
Pre‐miRNA
Inhibiting machinery
Dicer
Dicer
Drosha
Dicer
Drosha
Drosha
+
Dicer
Dicer
Dicer
Dicer
Drosha
Switch
ON
→
OFF
ON
→
OFF
OFF
→
ON
OFF
→
ON
ON
→
OFF
OFF
→
ON
ON
→
OFF
ON
→
OFF
OFF
→
ON
ON
→
OFF
Target gene
EGFP, DsRed
EGFP
EGFP
Luciferase
EGFP
EGFP, DsRed
EGFP, antiapoptotic gene (Bcl‐xL)
EGFP
EGFP, HIV‐1 Gag‐pol glycoprotein
Proapoptotic gene (FOXO1)
Cell type
HEK 293
HEK 293‐EGFP
HEK 293
HeLa
HEK293(T), HeLa, MDA‐MB‐231, HEK293T‐EGFP
HEK 293
HeLa, HeLa‐EGFP
HEK293FT
HeLa, MDA‐MB231, MDA‐MB231‐EGFP
MCF7