References 229BAP biotin acceptor peptide
LBT lanthanide binding peptide
TEV protease tobacco etch virus protease
PPV protease plum pox virus protease
TVMV tobacco vein mottling virus
CMP carbohydrate‐mimicking peptide
GST glutathione‐S‐transferase
Acknowledgment
I would like to thank Sven Panke for helpful discussion. The author was sup-
ported by a Junior Fellow award from the Simons Foundation.
References
1 Rizzuto, R. et al. (1995) Chimeric green fluorescent protein as a tool for
visualizing subcellular organelles in living cells. Curr. Biol., 5 (6), 635–642.
2 Lotze, J. et al. (2016) Peptide‐tags for site‐specific protein labelling in vitro and
in vivo. Mol. Biosyst., 12 (6), 1731–1745.
3 Pina, A.S., Batalha, I.L., and Roque, A.C. (2014) Affinity tags in protein purification
and peptide enrichment: an overview. Methods Mol. Biol., 1129 , 147–168.
4 Taxis, C. et al. (2009) Efficient protein depletion by genetically controlled
deprotection of a dormant N‐degron. Mol. Syst. Biol., 5 , 267.
5 Calles, B. and de Lorenzo, V. (2013) Expanding the Boolean logic of the
prokaryotic transcription factor XylR by functionalization of permissive sites
with a protease‐target sequence. ACS Synth. Biol., 2 (10), 594–603.
6 Pashov, A., Monzavi‐Karbassi, B., and Kieber‐Emmons, T. (2009) Immune
surveillance and immunotherapy: lessons from carbohydrate mimotopes.
Vaccine, 27 (25–26), 3405–3415.
7 Guenaga, J. et al. (2011) Heterologous epitope‐scaffold prime: boosting
immuno‐focuses B cell responses to the HIV‐1 gp41 2F5 neutralization
determinant. PLoS One, 6 (1), e16074.
8 Sun, Z.W. et al. (2016) An immunogen containing four tandem 10E8 epitope
repeats with exposed key residues induces antibodies that neutralize HIV‐1 and
activates an ADCC reporter gene. Emerg. Microbes Infect., 5 (6), e65.
9 Billerbeck, S. et al. (2013) Towards functional orthogonalisation of protein
complexes: individualisation of GroEL monomers leads to distinct
quasihomogeneous single rings. ChemBioChem, 14 (17), 2310–2321.
10 Zordan, R.E. et al. (2015) Avoiding the ends: internal epitope tagging of proteins
using transposon Tn7. Genetics, 200 (1), 47–58.
11 Sturgill, T.W. et al. (2008) TOR1 and TOR2 have distinct locations in live cells.
Eukaryot. Cell, 7 (10), 1819–1830.
12 Backstrom, M. et al. (1994) Insertion of a HIV‐1‐neutralizing epitope in a
surface‐exposed internal region of the cholera toxin B‐subunit. Gene, 149 (2),
211–217.