Synthetic Biology Parts, Devices and Applications

Table 12.2

(Continued)

Product

Enzyme

DNA‐binding protein and its target site

Chimeric protein

DNA scaffold

a)

Comments

References

Operators

No. units (n)

Spacer (bp)

Mevalonate

Acetoacetyl‐CoA thiolase (AtoB)

ZFaGTCGATGCC

AtoB‐ZFa (E1)

[1

:^1

:^ 1]

n

[1

:^2

:^ 1]

n

[1

:^2

:^ 2]

n

[1

:^4

:^ 1]

n

[1

:^4

:^ 2]

n

4, 8, 16

12

Up to threefold improvement in mevalonate production

[11]

Hydroxymethylglutaryl‐CoA synthase (HMGS)

ZFbGCGGCTGGG

HMGS‐ZFb (E2)

[1

:^1

:^ 1]

n

[1

:^2

:^ 1]

n

[1

:^2

:^ 2]

n

[1

:^4

:^ 1]

n

[1

:^4

:^ 2]

n

1, 2, 4, 8, 16

4

Hydroxymethylglutaryl‐CoA reductase (HMGR)

ZFcGAGGACGGC

HMGR‐ZFc (E3)

a)

Legend: [1

:^1

:^ 1]

repeats

spacer(bp) [E1‐spacer (bp)‐E2‐spacer (bp)‐E3‐spacer (bp)] repeated.