406 Index
p
pathway engineering 334
pathway libraries
Biobrick‐like assembly strategy 337
chromosomal integration 339
Gibson assembly method 335
plasmid assembly 340
pathway optimization 335, 337
pathways 331
peptides
antimicrobial 225
epitopes 224
functional 220
mimotopes 224
permissive sites in proteins 218
pharmaceutically relevant
functions 223
protein engineering 218
pharmaceutically relevant peptides
antimicrobial 225
epitopes 224
mimotopes 224
plasmid assembly
iterative multi‐step optimization
libraries 341
one‐step optimization libraries 340
pre‐initiation complex (PIC) 111
primary targeted deletions
defense systems 57
genes with unknown and exotic
functions 58
genetic diversity‐generating
factors 59
insertion sequences (ISs) 57
prophages 57
redundant and overlapping
functions 59
repeat sequences 58
virulence factors and surface
structures 58
prokaryotic platforms
B. subtilis platform 312
Chinese hamster ovary cell
extract 314
E.coli extract 311
eukaryotic platforms 312
insect cell extract 313Leishmania tarentolae extract 314
Streptomyces platform 312
wheat germ extract 313
Yeast extract 313
promoter 110
1,2‐propanediol 247
1,2‐propenediol 246
prophages 57
protein‐based organelles
bacterial microcompartments 283
biogenesis 289
chemical environment 288
Citrobacter freundii 288
minimal system 290
permeability 287
protein scaffold 224, 242
protein‐triggered RNA switches 174
public perceptions
in Austria 379
civil society groups 384
in European union 379
in Germany 381
in Netherlands 382
in United Kingdom 383
in U.S. 377r
RBS see ribosomal binding sites (RBS)
reactive peptides 223
Rec E/T system 17
recombinant technology 269
recombinases 42
recombineering substrate 17–18
recombineering systems 17
λ‐Red system 17–18
regulated promoters,” 113–115
regulated splicing 146
replication fork annealing model 17–18
repurposing existing organelles 293
responsible research and innovation (RRI)
definitions 389
ethics 394
European Union 389
gender equality 391
governance 395
open access approach 392
science education 392