408 Index
synthetic biology (SynBio) (contd.)
nanotechnology 387
new therapeutic paradigm 349
perceptions 377
replication 85, 87, 94, 96
reproduction 85, 87
RRI 389
T‐cell function 358
synthetic biology and splicing
impact of introns 150
splicing control by RNA devices 151
Synthetic Biology Open Language
(SBOL) 393
synthetic hybrid promoters
advantage 117
bipartite structure of natural
promoters 117
CRISPR‐derived system 121
DNA‐binding activity 119
heterologous transcription
factor 119
transcription activators 119
zinc fingers and TALE 119
synthetic Notch (synNotch)
receptors 356
synthetic organelles
in budding yeast 293
Citrobacter freundii 288
core design principles 282
De novo organelle 295
eukaryotes 281
lipid‐based organelles 292
permeability 282
prokaryotes 281
protein complexes 283
structure and function 282
synthetic promoters:
hybrid promoters,” 117–121
modified natural promoters,”
116–117
synthetic riboswitches 181, 183
synthetic RNA scaffolds
applications 268
catalytic roles of 262
dynamic 265
in vitro selection 264, 266
localizing metabolic enzymes 267in nature 263
packaging therapeutics 269
recombinant technology 269
research tools 266
secondary structure 262, 264
self‐assemble into
structure 264–265t
TAL‐DNA binding domains 249
targeted deletions
architectural studies 56
circular DNA‐based method 60
comparative genomics 56
CRISPR/Cas9 system 63
E. coli 59
gene essentiality 55
homologous recombination of
dsDNA 59
linear DNA‐based method 62
naturally evolved minimal
genomes 55
primary targets 57
in silico models 56
strategy for piling deletions 62
targeting oligos 19
TATA‐binding protein (TBP) 111
TATA element 111
T‐cell therapeutic function 357
trackable multiplex recombineering
(TRMR)
barcode identification 26
CREATE 27
dsDNA recombineering 26
experimental procedure 23
high throughput sequencing 24
library design and construction 19, 21
MAGE 27
mathematical modeling 26
microarray/sequencing analysis 24
overview 19–20
synthetic DNA cassette 19
validation 22
transcription activators 112
transcription factors 112
transcription initiation mechanism
112–113