Synthetic Biology Parts, Devices and Applications

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References 73

References


1 Arkin, A. (2008) Setting the standard in synthetic biology. Nat. Biotechnol., 26 ,
771–774.
2 Moon, T.S., Lou, C., Tamsir, A., Stanton, B.C. et al. (2012) Genetic
programs constructed from layered logic gates in single cells. Nature, 491 ,
249–253.
3 Gibson, D.G., Glass, J.I., Lartigue, C., Noskov, V.N. et al. (2010) Creation of
a bacterial cell controlled by a chemically synthesized genome. Science, 329 ,
52–56.
4 Csorgo, B., Feher, T., Timar, E., Blattner, F.R. et al. (2012) Low-mutation-rate,
reduced-genome Escherichia coli: an improved host for faithful maintenance of
engineered genetic constructs. Microb. Cell Fact., 11 , 11.
5 Umenhoffer, K., Feher, T., Balikó, G., Ayadin, F. et al. (2010) Reduced evolvability
of Escherichia coli MDS42, an IS-less cellular chassic for molecular and synthetic
biology applications. Microb. Cell Fact., 9 , 38.
6 Esvelt, K.M. and Wang, H.H. (2013) Genome-scale engineering for systems and
synthetic biology. Mol. Syst. Biol., 9 , 1–17.


0 1000 2000 3000 4000 5000

Fitness in natural environment

Obligate essential genes

Core genome Auxiliary genome

Gene no.

Genome streamlining

%

100

0

Fitness in controlled environment %

Figure 4.5 Hypothetical relationship between the fitness of the cell and the extent of genome
streamlining.

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