12
greater resolution to overturn or confi rm fundamental ideas as well as develop new
lines of investigation to pursue. Progress towards complete genome assemblies will
be a challenging but is an essential resource for future studies.
Developing new genetic tools to follow specifi c cellular movements, interactions
and contributions in all regenerating tissues is necessary to drive the model forward.
Particular focus will evolve towards identifying taxon-specifi c genes or molecules
with known orthologues through next-generation sequencing or candidate based
approaches. Emphasis will be placed on combining knock-down genetics with gain
of function assays unique to the salamander system to defi ne molecular mecha-
nisms. Research efforts in this area will enable the development of in vitro and
in vivo gain of function assays in mammals with the eventual goal of translating
these fi ndings for the treatment of human diseases and injuries.
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