Horticultural Reviews, Volume 44

Table 4.2.

List of most relevant cryopreservation studies with orchids.

Orchid species, cultivars,or hybrids

Explant type

Cryopreservationtechnique

z

Survival (%) ofexplants post-cryopreservation

References

Phalaenopsis

grex New

Toyohashi

Cell suspension

culture

Vitrification

Normal

protocormsrecovered

Tsukazaki et al. (2000)

Aerides odorata

Leaf segment

Encapsulation–

dehydration

• 
  

Hongthongkham and Bunnag

(2014)

Dendrobium

unregistered

hyb. (“Sena Red,” “MiniWRL”)

Pollen

Vitrification

≥

60%

Vendrame et al. (2008)

Cymbidium sinense

Pollen

Vitrification (4

◦C–6

◦C)

• 
  

Cheng (1984)

Phalaenopsis pulcherrima

(syn

. Doritis
pulcherrima

)

Protocorm

Vitrification PVS2

0%

Thammasiri (2000)

Dendrobium candidum

Protocorm

Air-drying, dehydration

• 
  

Bian et al. (2002)

Dendrobium cariniferum

Protocorm

Encapsulation–vitrification

20 min PVS2 (25

◦C

±

◦ 2

C)

15%

Pornnchuti and Thammasiri

(2008)

Dendrobium cruentum

Protocorm

Vitrification 240 min PVS2

(0

◦C

±

◦ 2

C)

33%

Thammasiri (2008)

Dendrobium cruentum

Protocorm

Encapsulation–

dehydration 50 minPVS2 (25

◦C

±

◦ 2

C)

27%

Thammasiri (2008)

Dendrobium cariniferum

Protocorm

Encapsulation–vitrification

60 min PVS2 (25

◦C

±

◦ 2

C)

15%

Thammasiri (2008)

Rhynchostylis gigantea

Protocorm

Vitrification 60 min PVS2

(25

◦C

±

◦ 2

C)

19%

Thammasiri (2008)