Horticultural Reviews, Volume 44

Table 4.2.

(Continued

)

Orchid species, cultivars,or hybrids

Explant type

Cryopreservationtechnique

z

Survival (%) ofexplants post-cryopreservation

References

Dendrobium nobile, D.

nobile

×

D.

nobile var.

nobilius

,D.

Lady

Hamilton

, Calanthe

furcate, CattleyaSuzanne Hye

×

C.

Bob

Betts,

Lc

. Corisande

×

Blc

.Norman’sBay

“Royal Bride,”

C.

Remy

Chollet

×

Lc

.Hyperion

Seed and pollen

Low temperature (

−^79

◦C)

(dehydrated)

• 
  

Ito (1965)

Cattleya

hyb. (undef.) and

Dendrobium nobile

Seed

Low temperature (

−^79

◦C)

(dehydrated orimmersed in glycerin)

Germinated well

(209 d and 465d)

Ito (1965)

Dendrobium phalaenopsis,

Phalaenopsis amabilis

Seed

Temperature (25

◦C–28

◦C) 30% (after 20 d)

Limartha (1975)

Calanthe

Takane and

C.

discolor

Seed

Low temperature (

−^15

◦C

and 17

◦C)

52%, 30% (after

1 year)

Hasegawa et al. (1978)

Gymnadenia conopsea,

Orchis morio, Ophrysapifera

Seed

Ultra low temperature

(−

196

◦C)

• 
  

Pritchard (1984)

Encyclia vitellinum

Seed

Low temperature (

−^40

◦C)

100% (5 weeks)

Koopowitz and Ward (1984)

Guarianthe aurantiaca

(syn.

Cattleya

aurantiaca

)

Seed

Temperature 20

◦C (with

water content of2.2 75.6%)

5% (after 1 year)

Seaton (1985)