- Varadarajan N, Julg B, Yamanaka YJ, Chen H,
Ogunniyi AO, McAndrew E, Porter LC,
Piechocka-Trocha A, Hill BJ, Douek DC,
Pereyra F, Walker BD, Love JC (2011) A
high-throughput single-cell analysis of
human CD8(+) T cell functions reveals dis-
cordance for cytokine secretion and cytolysis.
J Clin Invest 121(11):4322–4331.https://
doi.org/10.1172/JCI58653 - Han Q, Bagheri N, Bradshaw EM, Hafler DA,
Lauffenburger DA, Love JC (2012) Poly-
functional responses by human T cells result
from sequential release of cytokines. Proc
Natl Acad Sci U S A 109(5):1607–1612.
https://doi.org/10.1073/pnas.
1117194109 - Varadarajan N, Kwon DS, Law KM, Ogunniyi
AO, Anahtar MN, Richter JM, Walker BD,
Love JC (2012) Rapid, efficient functional
characterization and recovery of
HIV-specific human CD8+ T cells using
microengraving. Proc Natl Acad Sci U S A
109(10):3885–3890. https://doi.org/10.
1073/pnas.1111205109 - Yamanaka YJ, Berger CT, Sips M, Cheney PC,
Alter G, Love JC (2012) Single-cell analysis of
the dynamics and functional outcomes of
interactions between human natural killer
cells and target cells. Integr Biol 4
(10):1175–1184.https://doi.org/10.1039/
c2ib20167d - Yamanaka YJ, Szeto GL, Gierahn TM, Forcier
TL, Benedict KF, Brefo MS, Lauffenburger
DA, Irvine DJ, Love JC (2012) Cellular bar-
codes for efficiently profiling single-cell secre-
tory responses by microengraving. Anal
Chem 84(24):10531–10536. https://doi.
org/10.1021/ac302264q - Nguyen CQ, Ogunniyi AO, Karabiyik A,
Love JC (2013) Single-cell analysis reveals
isotype-specific autoreactive B cell repertoires
in Sjogren’s syndrome. PLoS One 8(3):
e58127. https://doi.org/10.1371/journal.
pone.0058127 - Torres AJ, Contento RL, Gordo S, Wucherp-
fennig KW, Love JC (2013) Functional
single-cell analysis of T-cell activation by sup-
ported lipid bilayer-tethered ligands on arrays
of nanowells. Lab Chip 13(1):90–99.
https://doi.org/10.1039/c2lc40869d - Torres AJ, Hill AS, Love JC (2014)
Nanowell-based immunoassays for measuring
single-cell secretion: characterization of trans-
port and surface binding. Anal Chem 86
(23):11562–11569. https://doi.org/10.
1021/ac4030297 - Fan R, Vermesh O, Srivastava A, Yen BK,
Qin L, Ahmad H, Kwong GA, Liu CC,
Gould J, Hood L, Heath JR (2008)
Integrated barcode chips for rapid, multi-
plexed analysis of proteins in microliter quan-
tities of blood. Nat Biotechnol 26
(12):1373–1378.https://doi.org/10.1038/
nbt.1507
- Shin YS, Ahmad H, Shi Q, Kim H, Pascal TA,
Fan R, Goddard WA 3rd, Heath JR (2010)
Chemistries for patterning robust DNA
microbarcodes enable multiplex assays of
cytoplasm proteins from single cancer cells.
Chemphyschem 11(14):3063–3069.
https://doi.org/10.1002/cphc.201000528 - Ahmad H, Sutherland A, Shin YS, Hwang K,
Qin L, Krom R-J, Heath JR (2011) A robot-
ics platform for automated batch fabrication
of high density, microfluidics-based DNA
microarrays, with applications to single cell,
multiplex assays of secreted proteins. Rev Sci
Instrum 82(9):094301.https://doi.org/10.
1063/1.3636077 - Ma C, Fan R, Ahmad H, Shi Q, Comin-
Anduix B, Chodon T, Koya RC, Liu CC,
Kwong GA, Radu CG, Ribas A, Heath JR
(2011) A clinical microchip for evaluation of
single immune cells reveals high functional
heterogeneity in phenotypically similar T
cells. Nat Med 17(6):738–743.https://doi.
org/10.1038/nm.2375 - Shin YS, Remacle F, Fan R, Hwang K, Wei W,
Ahmad H, Levine RD, Heath JR (2011) Pro-
tein signaling networks from single cell fluc-
tuations and information theory profiling.
Biophys J 100(10):2378–2386.https://doi.
org/10.1016/j.bpj.2011.04.025 - Vermesh U, Vermesh O, Wang J, Kwong GA,
Ma C, Hwang K, Heath JR (2011) High-
density, multiplexed patterning of cells at
single-cell resolution for tissue engineering
and other applications. Angew Chem 123
(32):7516–7518.https://doi.org/10.1002/
ange.201102249 - Shi Q, Qin L, Wei W, Geng F, Fan R, Shin YS,
Guo D, Hood L, Mischel PS, Heath JR
(2012) Single-cell proteomic chip for
profiling intracellular signaling pathways in
single tumor cells. Proc Natl Acad Sci U S A
109(2):419–424.https://doi.org/10.1073/
pnas.1110865109 - Wang J, Tham D, Wei W, Shin YS, Ma C,
Ahmad H, Shi Q, Yu J, Levine RD, Heath
JR (2012) Quantitating cell-cell interaction
functions with applications to glioblastoma
multiforme cancer cells. Nano Lett 12
(12):6101–6106.https://doi.org/10.1021/
nl302748q - Kwak M, Mu L, Lu Y, Chen JJ, Wu Y,
Brower K, Fan R (2013) Single-cell protein
308 Beiyuan Fan et al.