4 Dynamics of DNA Supercoiling 65Fig. 4.14.Inhibition of topoisomerase II activity measured by FLD technique.
Experimental conditions: Comparison of the kinetics of inhibition of reaction of
8 μg of pTM DNA with 4 u. of topoisomerase II by etoposide and AMPPNP. (a)
0 μM(curve1),5μM (2), 10μM (1), 25μM(2),50μM (3) of etoposide; (b)ratio
[AMPPNP]/([ATP]+[AMPPNP])=0 (curve 1), 0.005 (2), 0.01 (1), 0.02 (2)
of view the most prominent application of the developed approach is focused
on the dynamics of drug targeting, which can open up various biomedical
applications.
Acknowledgements
We would like to thank the Russian Foundation for Fundamental Investiga-
tions (00-04-48378, 05-01-00964) and Russian National Program of Support