Nature - USA (2020-01-16)

1 nature research | reporting summary October 2018 Corresponding author(s): Hai Qi Last updated by author(s):2019/10/19 Reportin ...

2 nature research | reporting summary October 2018 Field-specific reporting Please select the one below that is the best fit for ...

3 nature research | reporting summary October 2018 Animals and other organisms Policy information about studies involving animal ...

Nature | Vol 577 | 16 January 2020 | 421 Article Rapid non-uniform adaptation to conformation-specific KRAS(G12C) inhibition Jen ...

422 | Nature | Vol 577 | 16 January 2020 Article upon treatment with G12Ci) (Fig. 1c, Extended Data Fig. 3a, b). Treat- ment wit ...

Nature | Vol 577 | 16 January 2020 | 423 suggests that EGFR signalling is required for the escape from drug- induced quiescence. ...

424 | Nature | Vol 577 | 16 January 2020 Article of KRAS(G12C) is sufficient for the divergent response. However, drug-bound KRA ...

Nature | Vol 577 | 16 January 2020 | 425 Grün, D. & van Oudenaarden, A. Design and analysis of single-cell sequencing exper ...

Article Methods No statistical methods were used to predetermine sample size. The experiments were not randomized and investigat ...

quickCluster command. Cells in each cluster were normalized using a deconvolution method embedded in the scran package, which im ...

Article mean-variance relationship, which was then used to compute appropri- ate observation-level weight data. The data for eac ...

indicates that a gene is negatively selected. The fold change in expres- sion was also calculated to help interpret and filter t ...

Article is from Santa Cruz Biotechnology. Data analysis was performed with GraphPad Prism 7.0. Synergy determination To determin ...

Extended Data Fig. 1 | The effect of the G12Ci treatment on KR AS signalling across lung cancer cell lines. The indicated models ...

Article Extended Data Fig. 2 | Quality assessment and processing of scRNA-seq data. a, b, Gene counts as a function of UMI count ...

Extended Data Fig. 3 | KR AS(G12C)-dependent transcriptional output score in 10,177 lung cancer cells. a, The distribution of KR ...

Article Extended Data Fig. 4 | G12Ci treatment induces quiescence in a subpopulation of cancer cells. a, Single cells were analy ...

Extended Data Fig. 5 | Biosensor validation of the divergent response to G12Ci treatment. a, Cells expressing the quiescence bio ...

Article Extended Data Fig. 6 | Genes with trajectory-specif ic expression prof iles. a, Single cells were analysed to identify d ...

Extended Data Fig. 7 | The adaptive reactivation of KR AS during G12Ci treatment is dependent on EGFR signalling. a, The cells w ...