Nature - USA (2020-01-16)

Article Extended Data Fig. 8 | AURKA is involved in the reactivation of KR AS–GTP during G12Ci treatment. a, KR AS(G12C)-mutant ...

Extended Data Fig. 9 | Inhibition of MAPK signalling stimulates new KR AS synthesis. a, The cells were treated with the indicate ...

Article Extended Data Fig. 10 | Rapid non-uniform adaptation to conformation- specific KRAS(G12C) inhibition. Left, at baseline, ...

1 nature research | reporting summary October 2018 Corresponding author(s): Lito Last updated by author(s):09-27-19 Reporting Su ...

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3 nature research | reporting summary October 2018 Animals and other organisms Policy information about studies involving animal ...

426 | Nature | Vol 577 | 16 January 2020 Article FACT caught in the act of manipulating the nucleosome Yang Liu1,9, Keda Zhou1,9 ...

Nature | Vol 577 | 16 January 2020 | 427 the (H3–H4) 2 –DNA–FACT complex (Fig. 1c, d). In both structures, histones bind DNA and ...

428 | Nature | Vol 577 | 16 January 2020 Article Our map does not allow us to assign amino acids in FACT that directly participa ...

Nature | Vol 577 | 16 January 2020 | 429 that the disordered CTDs of one or both FACT subunits block DNA binding by preventing a ...

430 | Nature | Vol 577 | 16 January 2020 Article (Extended Data Fig. 8). Overall, this massive rearrangement allows SPT16 middl ...

Nature | Vol 577 | 16 January 2020 | 431 Both processes might be facilitated by the HMG domain of SSRP1^24 and other domains not ...

Article Methods Expression and purification of FACT and mutant FACT We infected Sf9 cells with Autographa californica multiple n ...

All conversions between Relion and CryoSPARC were performed using D. Asarnow’s pyem script (personal communication; https:// git ...

Article Author contributions Y.L. cloned and expressed all FACT constructs with the help of K.Z. K.Z. and Y.L. optimized sample ...

Extended Data Fig. 1 | Analysis of electron-microscopic data. a, Representative micrograph of particles containing 79 bp of 601 ...

Article Extended Data Fig. 2 | Validation of electron-microscopic data. a, Local- resolution map and FSC curve for class 1 (comp ...

Extended Data Fig. 3 | Electron density and models. a, Density maps for class 1 and 2 particles in three orthogonal orientations ...

Article Extended Data Fig. 4 | Coverage maps from FACT HDX experiments. a, b, SPT16 and SSRP1 coverage maps from HDX experiments ...

Extended Data Fig. 5 | Changes in deuterium uptake mapped onto complex 1. Deuterium-uptake plots are included for example peptid ...