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nature research | reporting summary
April 2018mCherry (Clontech, Cat #631987). Plasmids generated specifically for this publication are available through authors upon
request. These materials will also be available through Addgene upon publication of this manuscript.Antibodies
Antibodies used Antibody name Vendor Catalog # Lot # Dilution
Rabbit monoclonal anti-E-cadherin Cell Signaling Technology Cat #3195 Lot #13 1:1000
Rat monoclonal anti-E-cadherin Abcam Cat #ab11512 Lot #GR183496-1 1:1000
Rat monoclonal anti-E-cadherin Sigma Cat #U-3254 Lot #077M4800V 1:1000
Rabbit polyclonal anti-ZEB1 Santa cruz Cat #sc-25388 Lot #unknown 1:1000
Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat #8685 Lot #6 1:1000
Rabbit monoclonal anti-HA-Tag Cell Signaling Technology Cat #3724 Lot #unknown 1:1000
Mouse monoclonal anti-FLAG M2 Sigma Cat #F1804 Lot #SLBT7654 1:2000
Rabbit polyclonal anti-RREB1 Genway Biotech Cat #GWB-5B0668 Lot #27022 1:500
Mouse monoclonal anti-α-Tubulin Sigma Cat #T6199 Lot #unkown 1:10,000
Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat #9661 Lot #43 1:1000
Goat polyclonal anti-Brachyury R&D Cat #AF2085 Lot #unkown 1:200
Rabbit polyclonal anti-phospho-H3 Millipore Cat #06-570 Lot #6570 1:300
Rabbit polyclonal anti-N-cadherin Santa cruz Cat #sc-7939 Lot #unkown 1:300
Rabbit polyclonal anti-RFP Rockland Cat #600-401-379 Lot #42393 1:300
Goat polyclonal anti-SNAIL R&D Cat #AF3639 Lot #XRS0218061 1:100
Rat monoclonal anti-SOX2 eBioscience Cat #14-9811-82 Lot #2023691 1:200
Rabbit polyclonal anti-alpha SMA Abcam Cat #ab5694 Lot #GR248336-4 1:1000
Rabbit monoclonal [Y92] anti-PDGFR beta Abcam Cat #ab32570 Lot #GR212663-49 1:1000
Mouse monoclonal anti-β-Actin Cell Signaling Technology Cat #3700 Lot #unknown 1:1000
Donkey anti-rabbit alexa 568 Invitrogen Cat #A10042 Lot #1964370 1:500
Donkey anti-goat alexa 488 Invitrogen Cat #A11055 Lot #830720 1:500
Donkey anti-rat dylight 650 Invitrogen Cat #SA5-10029 Lot #UF2789721 1:500
Donkey anti-rat alexa 488 Invitrogen Cat #A21208 Lot #1932496 1:500
Donkey anti-rabbit alexa 647 Invitrogen Cat #A31573 Lot #1903516 1:500
Donkey anti-rabbit alexa 488 Invitrogen Cat #A21206 Lot #2045215 1:500Validation Validation statement for each primary antibody is provided on the manufacture's website.Eukaryotic cell lines
Policy information about cell lines
Cell line source(s) Mouse KSIC PDA cell line was provided by Nabeel Bardeesy. Mouse CIY pancreatic organoid lines were generated from CIY
mice. Mouse lung epithelial cell line 393T3 cell line was a gift from Taylor Jacks. Other cell lines (human lung epithelial cell
line A549, mouse mammary gland epithelial cell line NMuMG and mouse embryonic stem cell line E14Tg2a.IV) were
purchased from ATCC.Authentication Mouse KSIC PDA cell line, CIY pancreatic organoid lines and 393T3 cell line were genotyped by PCR amplification. A549 cells
were 100% authenticated with STR profiling. NMuMG and E14Tg2a.IV cells were validated with RNA-seq analysis for
signature gene expression profile.Mycoplasma contamination All cell lines tested negative for mycoplasma contamination.Commonly misidentified lines
(See ICLAC register)None of the cell lines used are listed as commonly misidentified lines in the ICLAC database.Animals and other organisms
Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research
Laboratory animals Pdx1-Cre; Cdkn2a -/-;LSL-YFP (CIY) and Pdx1-Cre; KrasG12D+/-; Cdkn2a-/-; Smad4-/- (KSIC) mice were provided by Nabeel
Bardeesy. FVB/NJ (strain 001800) and B6129SF1/J (strain 101043) mouse strains were obtained from the Jackson Laboratory.
Athymic nude mice (Hsd:Athymic Nude-Foxn1nu, 069) were obtained from Envigo. For all cancer cell injection studies, female
mice were used between ages 5-7 weeks of age. All animal experiments were conducted in accordance with protocols approved
by the MSKCC Institutional Animal Care and Use Committee and were in compliance with the relevant ethical regulations
regarding animal research.Wild animals The study did not involve wild animals.Field-collected samples The study did not involve samples collected in the field.