Nature - USA (2019-07-18)

reSeArcH Article

HSPC WT

HSPC MUT

MkP WT

MkP MUT

(n = 19)

(n = 16)

(n = 17)

(n = 51)

(n = 108)

(n = 240)

(n = 25)

(n = 276)

(n = 14)

(n = 23)

(n = 14)

(n = 103)

(n = 701)

(n = 149)

(n = 229 )

(n = 125 )

2

3

4

5

Log

(ATF6 module) 2

4

5

3

4

4

5

3

4

3

4

4

55

4

5

2

3

4

5

4

6

5

ET01 ET02 ET03 ET04 ET05

P = 4.6 x 10-5

P = 1.4 x 10-6

P = 0.22

P = 0.082

P = 0.42

P = 1.1 x 10-5

P = 0.49

P = 0.0021

P = 0.0012

P = 6.9 x 10-5

Log

(PERK module) 2

1

2

3

1

2

0

1

2

3

4

1

2

3

1

2

3

1

2

3

1

2

3

1

2

3

1

2

3

4

0

1

2

3

4

ET01 ET02 ET03 ET04 ET05

P = 0.29

P = 0.037

P = 0.7

P = 0.82

P = 0.41

P = 0.94

P = 0.4

P = 0.41

P = 0.51

P = 0.38

3

4

5

Log

(XBP1 module) 2

3

4

5

4

5

4

5

4

5

4

5

4

5

4

5

4

5

4

5

6

ET01 ET02 ET03 ET04 ET05

P = 0.0097

P = 8.6 x 10-6

P = 0.17

P = 0.015

P = 0.022

P = 0.0022

P = 0.1

P = 0.0038

P = 0.011

P = 4.7 x 10-4

(n = 862)

(n = 185)

(n = 157)

(n = 85)

ET01 ET02 ET03 ET04 ET05

HSPC WT

HSPC MUT

MkP WT

MkP MUT

Extended Data Fig. 7 | ATF6 and IRE1 branches of the UPR are
activated in CALR-mutated progenitor cells. By sample (ET01–ET05),
expression of ATF6-, PERK- and XBP1-target genes in the UPR in CALR

wild-type and mutant MkPs and HSPCs (two-sided Wilcoxon rank-sum

test).