Nature - USA (2020-08-20)

474 | Nature | Vol 584 | 20 August 2020 Article The existence of this tradeoff suggests that it might be advanta- geous for cell ...

Methods Descriptions herein refer to a shift to acetate, but the same methods also apply to other shifts. No statistical methods ...

Article B. thetaiotaomicron, the pH changes were from 7.2 (fresh anoxic me- dium) to 6.9 (OD 600 approximately 0.4). The pH for ...

stressful conditions and poor feeding) or which were out of focus were discarded. Out of a total of 1,494 starting trenches, 363 ...

Article the cell divided, or the field of view became obstructed by adjacent dividing cells, or the cell became dislodged from t ...

as were peptides of fewer than six residues. Spectral libraries for each each condition were built and refined using Spectrast ( ...

Article Extended Data Fig. 1 | Growth curves for shifts. a, Growth curves following shifts from different glycolytic carbons to ...

Extended Data Fig. 2 | Lag-time/growth-rate relations. a–f, The inverse of lag times following a shift to the indicated sugars ( ...

Article Schematic of the microfluidic device inlet outlet Mother-Machine chip media flow mother cell preshift glucose postshift ...

Extended Data Fig. 4 | Single-cell behaviour during a glucose-to-acetate shift through time-lapse microscopy of batch culture. a ...

Article Extended Data Fig. 5 | Absolute and relative concentrations of key metabolites in the shift from glucose to acetate. a, ...

Extended Data Fig. 6 | See next page for caption. ...

Article Extended Data Fig. 6 | Proteomics-based characterization of lag-phase dynamics. a–f, Gluconeogenic enzymes. Relative lev ...

Extended Data Fig. 7 | See next page for caption. ...

Article Extended Data Fig. 7 | Sequential f lux limitation model and trade-off between growth and lag. a, Intuitively, in our mo ...

Extended Data Fig. 8 | Preshift overexpression of glycolytic enzymes. a–d, Lag times following shifts from glucose to: a, acetat ...

Article Extended Data Fig. 9 | Improved growth of Cra-knockout E. coli, and trade-offs for other microbes. a, Growth rates of th ...

Extended Data Fig. 10 | Optimal growth rate as a function of the expected substrate abundance in an environment. a, Cells initia ...

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