Nature - USA (2020-08-20)

Extended Data Fig. 2 | Lag-time/growth-rate relations.
a–f, The inverse of lag times following a shift to the indicated
sugars (a, to acetate; b, to pyruvate; c, to succinate; d, to
fumarate; e, to lactate; f, to malate) is plotted as a function of
the preshift growth rate in glycolytic conditions. The preshift
growth rate was modulated using different carbon sources
(circles) and through lactose-uptake titration (squares). Solid
lines show nonlinear least-squares fits (Matlab lsqcurvefit
function) of lag times as a function of preshift growth rates
according to the relation given by equation ( 1 ). Most lag
phases agree very well with equation ( 1 ); only a few shifts,
with short lag times (low growth rates), deviate somewhat
from this relation. This is partly the result of plotting inverse
lag times, which amplifies relatively small experimental
variations in lag times for short lag phases. These fits allow us
to estimate 95% confidence intervals for model parameters
(Matlab nlparci function), most importantly for the critical
growth rates λ 0. For acetate, λC = (1 .10 ± 0.01) h−1,
α = 0.78 ± 0.10, n = 17; for pyruvate, λC = (1 .1 2 ± 0.03) h−1,
α = 0.33 ± 0.07, n = 17; for succinate, λC = (1 .1 3 ± 0.0 4) h−1,
α = 0.33 ± 0.10, n = 14; for fumarate, λC = (1 .08 ± 0.02) h−1,
α = 0.23 ± 0.07, n = 5; for lactate, λC = (1 .09 ± 0.05) h−1,
α = 0.22 ± 0.15, n = 5; for malate, λC = (1 .17 ± 0.09) h−1,
α = 0.22 ± 0.11, n = 5. g, Lag times as a function of steady-state
growth rates in the postshift medium for different preshift
media. Coloured solid lines show linear regressions of the
corresponding coloured data points. Carbon sources that
allow slower growth rates tend to result in longer lag phases
when they are the postshift carbon sources. This intuitive
correlation has previously been characterized^13.