Nature - USA (2020-08-20)

Nature | Vol 584 | 20 August 2020 | 481 mutant produced only a modest phenotype, highlighting the promi- nent multipoint backbon ...

482 | Nature | Vol 584 | 20 August 2020 Article Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa (Table ...

Nature | Vol 584 | 20 August 2020 | 483 PbgA presents a new paradigm in selective lipid recognition as it does not seem to requi ...

Article Methods Bacterial strains and plasmids To generate pBAD-pbgA, pbgA was amplified from uropathogenic E. coli (UPEC CFT073 ...

(GE Healthcare) at 1:5,000 dilution was incubated with the nitrocel- lulose for 1 h in 5% non-fat milk in TBS plus 0.05% Tween 2 ...

Article oven and autosampler were set at 45 °C and 15 °C, respectively. Flow rate was 0.3 ml min−1 and the gradient was held at ...

Coherent Light Source at SLAC National Accelerator Laboratory. 7.9 MAG was added to the PbgA microcrystals LCP medium at around ...

Article suspension was sonicated for 5 min and then left in a boiling water bath for 30 min to cleave the O-antigen from Kdo 2 - ...

(Taxonomy 83333, downloaded 1 July 2017), known contaminants and the reversed versions of each sequence. Peptide assignments wer ...

Article b 10 -3 10 -2 10 -1 100 101 102 0.1 0.4 0.03 rifampicin (uM) OD 600 K-12 K-12 pBADpbgA pbgA-pBADpbgA + ind. pbgA-pBADpbg ...

a b 68 kDa E.coli PbgA molar mass (kDa ) 0 250 500 50 75 100 7.07.5 8.08.5 A^280 Time (min) 71 kDa S.typhimurium PbgA molar mass ...

Article Extended Data Fig. 2 | Biophysical and structural characterization of PbgA. a, E. coli and S. typhimurium PbgA were puri ...

abb equilibrate production (500 ns)equilibrateproduction (500 ns) Run 1A: starting from 2.0 Å X-ray LPS-PbgA Run 2A: starting fr ...

Article Extended Data Fig. 3 | PbgA structural alignments and molecular dynamics simulations. a, Structural superposition of Pbg ...

a b + cardiolipin + LPS Lnt PbgA tetra-acylated lipid A hexa-acylated lipid A tetra-acylated monophosphoryl lipid A hexa-acylate ...

Article TMD E.clocacae K.pneumoniae S.typhimurium Y.pestis V.cholerae PH.influenz.rustigianiia S.frigidimarina V.parahaemolyticu ...

a M212AM212EM212KT213VT213DR215AR216AR216ER216DM212A/ T213V/ R216A vector WT WT vector D268AT302VR451A b +WT PbgA +D268A +T302A ...

Article a c LPS CL PG PE 1 0 1 0 1 0 1 0 response (nm) LABWT LABWT+ LABT213D 0 150 100 50 25 10 KD (LPS) LABWT 73±13μM LABWT+ 52 ...

a c PbgA-prey PbgA-bait LapB-bait HisM-baitFtsH-bait b + LapB + PlsY E. coli K-12 ΔpbgA::pBADpbgA + LapB + PlsY 0.02% arabinose ...

Article Extended Data Fig. 8 | PbgA interacts with LapB to regulate LpxC stability. a, Proteins identified by mass spectrometry ...