Nature - USA (2020-08-20)

2

nature research | reporting summary

April 2018

Data

Policy information about availability of data

All manuscripts must include a data availability statement. This statement should provide the following information, where applicable:

• Accession codes, unique identifiers, or web links for publicly available datasets


• A list of figures that have associated raw data


• A description of any restrictions on data availability
  The authors declare that all data supporting the findings of this study are available within the paper and the Supplementary Information files. Raw reads for RNA
  sequencing data are available at the European Nucleotide Archive under the study accession PRJEB36386.

Field-specific reporting

Please select the best fit for your research. If you are not sure, read the appropriate sections before making your selection.

Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

For a reference copy of the document with all sections, see nature.com/authors/policies/ReportingSummary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size No statistical method was used to predetermine sample size. For each experiment the n values were reported in the legend, source data file

and Extended Data Tables 1-3. We used standard sample sizes based on previous experiments and literature and that were needed to

perform the statistical tests.

Data exclusions In general, data were not excluded. For aggregation counting, all worms that died during the experiments, crawled off the plate, exploded or

bagged were not included in the data (pre-established criteria). For lifespan assay, worms that crawled off the plate, exploded or bagged

were not included in the data (pre-established criteria). For survival assay, worms that crawled off the plate or exploded were not included in

the data (pre-established criteria). This exclusion criteria is extensively used in the field.

Replication Experiments were repeated at least two times. All results were successfully and reliably repeated (see source data with repeats). Effects of top

13 ECRs were independently confirmed by two investigators.

Randomization Worms were randomly distributed into treatment and control groups.

Blinding Investigators were blinded to the genotype or RNAi treatments when feasible. For obvious mutant phenotypes or for strong overexpression of

ECRs visible in the red channel of the fluorescence dissecting microscope, this was not possible. Survival and lifespan assays were not

performed blind as death is an unambiguous read-out.

Reporting for specific materials, systems and methods

Materials & experimental systems

n/a Involved in the study

Unique biological materials

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Antibodies

Antibodies used Anti-tRFP, Evrogen, cat.# AB233, Lot. 23301301013, dilution 1:1000 and 1:4000.

anti-His H3, Santa Cruz Biotechnology, cat.# sc-8036, Lot. 10414, dilution 1:2000.

Secondary anti-rabbit IRDye680RD, LI-COR, cat.# 927-68071, Lot. C51104-08, dilution 1:10,000.

Validation http://evrogen.com/products/antibodies/AB-tRFP.shtml, https://www.scbt.com/scbt/product/his-probe-antibody-h-3