Extended Data Fig. 3 | Flow cytometry gating strategy. a, Forward scatter
area (FSC-A) versus forward scatter height (FSC-H) density plot for doublet
exclusion. b, Forward and side scatter (SSC-A) density plots to identify the
lymphocyte population. c, Live T cells were gated based on CD3 expression and
a live/dead discrimination dye. d, e, Only single expressing CD8 and CD4 T cells
were Boolean gated (d) and used for IFNγ and/or TNF analysis (e).