Extended Data Fig. 6 | T-DNA insertion lines used in this study and
transcript levels. a, Gene structure of OSCA1. 3 and OSCA1.7 showing exons
(black boxes) and introns (lines) as well as location of T-DNA insertions. Line
osca1.3/1.7 was obtained by crossing osca1.3 and osca1.7. Arrows denote
location of primers used for genotyping. b, Transcript levels of OSCA1. 3 and
OSCA1.7 in Col-0, osca1.3, osca1.7 and osca1.3/1.7 as determined by quantitative
real-time PCR with reverse transcription. Values are mean +/− s.d. (n = 6,
representing 2 independent experiments with 3 biological repeats each).
c, Transcript levels of OSCA1. 3 in Col-0, osca1.3/1.7 and osca1.3/1.7
complemented with OSCA1. 3(WT) or OSCA1.3(S54A), respectively. Expression
levels for three independent T1 plants corresponding to Fig. 4f are shown
separately, with two technical replicates (leaves). This experiment was
repeated three times. Shown are quantitative real-time RT–PCR data relative to
U-box (At5g15400). Primers used in b and c are listed in Supplementary Table 2.