Soil Biology and Ecology
Unit 2.3 | 31
Demonstration 3: Assessing Earthworm
Populations as Indicators of Soil Quality
for the instructor
Overview
This demonstration introduces
students to techniques for assessing
earthworm populations as indicators
of soil quality.
You have a choice of two methods
for this demonstration. The shovel-
count method will be more tedious
for the students because they
will have to sort through the soil
and remove all earthworms. The
vermifuge method may take a little
more effort at first to gather the
materials needed, but it will make
the students’ work easier.
MateriaLs
SHOvEl-COUNT METHOD
• Shovels
• Earthworm Data Sheets (appendix 5)
• Pencils
vERMIFUGE METHOD
• Sample rings*
• Clippers
• Watering can
• Scoop
• Stirring rod
• Fresh water
• Jars
• Earthworm Data Sheets (appendix5)
• Pencils
• Ground yellow mustard seed (available in bulk from
health food stores or from herb companies)**
*Sample rings define the sample area and prevent vermi-
fuge from escaping sample area. A simple design is to cut
the top 8–12 inches from a 5-gallon drum and weld on a
piece of metal pipe that overhangs each side by 6 inches
to use as a handle. The ring is pressed into the soil to 2–3
inches depth, and vermifuge is added within the sample
ring. Sample rings can also be fashioned from sheet metal,
housing duct pipes, or large clean paint cans with the bot-
tom cut off.
**60 ml (volume) or 32 grams of yellow mustard powder
to 4.5 liters of tap water = 13 ml/1 liter or 7g/liter.
4.5 liters of vermifuge is the amount required per sample
area in this demonstration.
Instructor’s Demonstration 3 Outline