Methods in Molecular Biology • 16 Enzymes of Molecular Biology

50 Landgraf and Wolfes In the plateau phase of the reaction, the processivity of Taq poly- merase decreases at excess template c ...

Taq Polymerase % faultless Sequences 100 51 80 60 40 20 I I I I I 1 2 3 4 5 [log] Amplification Fig. 7. Dependence of error rate ...

52 Landgraf and Wolfes The enzyme is highly processive; Secondary structures are suppressed at the optimal temperature range of ...

Taq Polymerase 53 Mullis, K. B. (1989) The polymerase chain reaction: why it works, in Current Communications in Molecular Biol ...

54 Landgraf and Wolfes Smith, L. M., Sanders, J. Z., Kaiser, R. J., Huges, P., Chris, D., Heiner, C., Kent, S. B. H., and Hood, ...

Taq Polymerase 55 Sherman, D. R., Geliebter, J., and Cross, G. A. M. (1989) Rapid and simple amplification of a specific RNA se ...

56 Landgraf and Wolfes Bos, J. L., Fearon, E. R., Hamilton, S. R., Verlan-de Vries, M., van Boom, J. H., van der Eb, A. H., and ...

Taq Polymerase 57 Lomeli, H., Tyagi, S., Pritchard, C. G., Lizardi, P. M., and Kramer, F. R. (1989) Quantitative assays based o ...

58 Landgraf and Wolfes Mole, S. E., Iggo, R. D., and Lane, D. P. (1989) Using the polymerase chain reaction to modify expressio ...

CHAPTER 5 Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6) Deborah tL Cook and Robert J. Slater Introduction DNA-dependent RNA p ...

60 Cook and Slater template and interact with protein factors that modulate the activity of the enzyme. Several reviews have app ...

Eukaryotic RNA Polymerases Table 1 Properties of Eukaryotic RNA Polymerases a 61 Eukaryotic RNA polymerases I II III Cellular lo ...

62 Cook and Slater 2.2. Ion Requirement The requirement for a divalent cation by RNA polymerases indicates binding of nucleotide ...

Eukaryotic RNA Polymerases 63 cally interacts with RNA polymerase II and TFIIB (26,27). These transcription factors range in mol ...

64 Cook and Slater strated accurate transcription of exogenous DNA by polymerase III, and others such as those developed by Weil ...

Eukaryotic RNA Polymerases 65 20-30 gL Whole-cell or nuclear extract; 1-2.5 lag Template DNA; 500 I.tM each of ATP, CTP, and UT ...

66 Cook and Slater system (with the omission of the radiolabeled triphosphate, as described in Section 4.2.) and hybridized with ...

Eukaryotic RNA Polymerases 67 mixed together, resuspended in hybridization buffer (40 mM PIPES [pH 6.4], 1 mM EDTA [pH 8.0], 0.4 ...

68 Cook and Slater RNA polymerase II. RNA polymerase II from wheat germ does not function in this system, indicating that it is ...

Eukaryotic RNA Polymerases 69 NaC1 appears to release all of the nuclear components required for specific transcription, includi ...