Methods in Molecular Biology • 16 Enzymes of Molecular Biology

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Enzymes of Molecular Biology ...

Methods in Molecular Biology John M. Walker, SER ES EDitOR Enzymes of Molecular Biology, edited by Michael M. Burrell, 1993 PCR ...

r • Methods in Molecular Biology • 16 Enzymes of Molecular Biology Edited by Michael M. Burrell Advanced Technologies (Cambridge ...

© 1993 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512 All rights reserved. No part of this book may b ...

Preface The scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This ...

vi Preface provides details on the size and structure of the protein. This is followed by a discussion of those specific paramet ...

Contents Preface ............................................................................................................... ...

viii Contents Crt. 15. CH. 16. CH. 17. CH. 18. CH. 19. CH. 20. Proteolytic Enzymes for Peptide Production, Patricia J. Sweeney a ...

Contributors JORGEN ALVES ° Medizinische Hochschule Hannover, Zentrum Biochemie, Abteilung Biophysikalische Chemie, Hannover, Ge ...

x Contributors MARTIN J. MAUNDERS • Advanced Technologies (Cambridge) Ltd., Cambridge, UK ALFRED PmGOUD • Medizinische Hochschul ...

CHAPTER 1 Nucleases An Overview A. Fred Weir Enzymes able to digest nucleic acids are of course essential to molecu- lar biology ...

2 Weir cleases (DNases) and then to focus on the techniques in which they are used. The enzymes that molecular biologists use as ...

Nucleases--An Overview 3 Table 1 The Nomenclature of Nuclease EC number Reaction Example 3.1. I 1 Exodeoxyribonucleases producin ...

4 Weir 1.1.1. Criteria Used for Classification 1.1.1.1. Exo- vs ENDONUCLEOLYTIC CLEAVAGE Exo-DNases cleave from the ends of DNA ...

Nucleases--An Overview 5 1.1.1.4. CLEAVAGE OF NATIVE OR SINGLE-STRANDED DNA Nucleascs tcnd to havc a"prcfcrcnce" for cleavage of ...

6 Weir Felsenfeld, G. (1978) Chromatin. Nature 271, 115-121. Kroeker, W. D. and Fairley, J. L. (1975) Specific limited cleavage ...

CHAPTER 2 Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1) A. Fred Weir Introduction From Chapter 1 on nucleases, we know ...

8 Weir has been commercially available for at least 25 years, most of the work on the properties of the enzyme being done in the ...

Deoxyribonucleases of Molecular Biology A 9 3' 5' 5' 5'P. 5*P 5°P 3' 3' 5'P 5' 5' 5'P 5'P 5'P 3' 3' 5'P 5'P 5'P ~5'P .5' B 5, .3 ...