Methods in Molecular Biology • 16 Enzymes of Molecular Biology

(Nancy Kaufman) #1

Ligases 227



  1. Murray, N. E., Bruce, S. A., and Murray, K. (1979) Molecular cloning of the
    DNA ligase gene from bacteriophage T4. II. Amplification and preparation of
    the gene product. J. Mol. Biol. 132, 493-505.

  2. Modrich, P., Anraku, Y., and Lehman, I. R. (1973) Deoxyribonucleic acid
    ligase. Isolation and physical characterization of the homogeneous enzyme
    from Escherichia coli. J. Biol. Chem. 248, 7495-7501.

  3. Knopf, K. W. (1977) Simple isolation method and assay for T4 DNA ligase
    and characterization of the purified enzyme. Eur. J. Biochem. 73, 33-38.

  4. Higgins, N. P. and Cozzarelli, N. R. (1980) DNA joining enzymes: a review.
    Methods Enzymol. 68, 50-71.

  5. Weiss, B., Jacquemin-Sablon, A., Live, T. R., Fareed, G. C., and Richardson,
    C. C. (1968) Enzymatic breakage and joining of deoxyribonucleic acid. VI.
    Further purification and properties of polynucleotide liase from Escherichia
    coli infected with bacteriophage T4. J. Biol. Chem. 243, 4543--4555.

  6. Sgaramella, V. and Khorana, H. G. (1972) Studies on polynucleotides. CXII.
    Total synthesis of the structural gene for an alanine transfer RNA from yeast.
    Enzymic joining of chemically synthesized polynucleotides to form the DNA
    duplex representing nucleotide sequence 1 to 20. J. Mol. Biol. 72, 427-444.

  7. Shortle, D., Griszfi, P., Benkovic, S. J., and Botstein, D. (1982) Gap misrepair
    mutagenesis: efficient site-directed induction of transition, transversion, and
    frameshift mutations in vitro. Proc. Natl. Acad. Sci. USA 79, 1588-1592.

  8. Modrich, P., Lehman, I. R., and Wang, J. C. (1972) Enzymatic joining of poly-
    nucleotides. XI. Reversal of Escherichia coli deoxyribonucleic acid ligase reac-
    tion. J. Biol. Chem. 247, 6370-6372.

  9. Nath, K. and Hurwitz, J. (1974) Covalent attachment of polyribonucleotides
    to polydeoxyribonucleotides catalyzed by deoxyribonucleic acid ligase. J. Biol.
    Chem. 249, 3680-3688.

  10. Kleppe, K., Van de Sande, J. H., and Khorana, H. G. (1970) Polynucleotide
    ligase catalysed joining of deoxyribo-oligonucleotides on ribopolynucleotide
    templates and of ribo-oligonucleotides on deoxyribopolynucleotide templates.
    Proc. Natl. Acad. Sci. USA 67, 68-73.

  11. Modrich, P. and Lehman, I. R. (1973) Deoxyribonucleic acid ligase. A steady
    state kinetic analysis of the reaction catalysed by the enzyme from Escherichia
    coli. J. Biol. Chem. 248, 7502-7511.

  12. Sugino, A., Goodman, H. M., Heynecker, H. L., Shine, J., Boyer, H. W., and
    Cozzarelli, N. R. (1977) Interaction of bacteriophage T4 RNA and DNA ligases
    in joining duplex DNA at base paired ends. J. Biol. Chem. 252, 3987-3994.

  13. Olivera, B. M. and Lehman, I. R. (1967) Diphosphopyridine nucleotide: A
    cofactor for the polynucleotide joining enzyme from Escherichia coli. Proc.
    Natl. Acad. Sci. USA 57, 1700-1704.

  14. Hinkle, D. C. and Richardson, C. C. (1975) Bacteriophage T7 deoxyribonucleic
    acid replication in vitro. Purification and properties of the gene 4 protein of
    bacteriophage T7. J. Biol. Chem. 250, 5523-5529

  15. Dugaiczyk, A., Boyer, H. W., and Goodman, H. M. (1975) Ligation of EcoRI
    endonuclease generated DNA fragments into linear and circular structures. J.
    Mol. Biol. 96, 171-184.

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