Index 367
DNA cellulose, 111
DNA fingerprint, 181
dyes, 124
eicosomer, 111
electrophoresis, 126, 127, 174, 175
enzymoleogy, 109, 110
fill in reaction, 183
fragment pattern stability assay, 163,
164
gapped duplex protocol, 184, 185
heparin agarose, 110
homochromatography, 174
homologies, 111-113
hydroxyapatite, 110
inhibition, 123, 124
isoschizomer, 111,175
Kcat-values, 116
KM-Values, 116
ligation recut assay, 163, 165
methylation, 121,166-173, 175, 181
methyltransferase, 120, 121,166-173
mis-match, 185
modification, 107, 108, 110, 123, 124,
166-173
modification enzymes, 107-109, 121,
167
molecular properties, 111-113
n-ethyl maleimide, 123, 124
netropsin, 124
nomenclature, 109
oligodeoxynucleotides, modified, 120
oligonucleotides, 111
olivomycin, 123, 124
optimum reaction conditions, 128-163
order of reaction, 118, 119
overhang, 110
p-mercuribenzoate, 123, 124
phosphatase, 183
phosphocellulose, 110
phosphorothioate, 118
plasmid nicking assay, 163, 164
polynucleotide kinase, 183
preparative applications, 184, 185
proteinase K, 166
purification, 110, 111
rate of reaction, 117, 118reaction mechanisms, 113-119
recognition sequences, 130-162
restriction fragment length polymor-
phism (RFLP), 180
restriction map, 175
S-adenosylmethionine (SAM), 108
sequences, 111-113
site directed metagenesis, 184, 185
specific activity, 125, 126
specificity, 119-123
"star" activity, 121,122, 174
"star" pattern, 122, 174
sticky ends, 110
storage, 124, 125
subunit composition, 112, 113
sulfhydryl reagents, 123, 124
suppliers, 180
temperature optimum, 117
terminal deoxynucleotidyl transferase,
183
three dimensional structures, 113, 120,
185, 186
Type I, II, II, 108
Reverse transcriptase, 73-94
AMV RT, 73, 74
catalytic properties, 76
DNA polymerase, 76
E. coli DNA polymerase, 82
E. coli RNase H, 82
E. coli DNA ligase, 82
enzyme, 74
error rate, 76
experimental procedures, 83
DEPC, 84
first strand reaction, 85
one tube double-stranded cDNA
synthesis, 85
product analysis, 87
second strand reaction, 86
M-MLVH-RT, 74
oligo(dT)12_18, 78
processivity, 76
random hexamers, 78
reaction conditions, 79
actinomycin D, 81
bovine serum albumin, 80