Methods in Molecular Biology • 16 Enzymes of Molecular Biology

368 Index

dNTPs, 79
inhibitors, 81
Mg 2÷, 79
sodium pyrophosphate, 80
pH and metal ion, 79
polyanions, 80
reducing agent, 81
RNase inhibitors, 80
temperature, 81
retroviral, 73
RNase H, 73, 74
RNase H, 76
second strand reaction, 82
secondary structure-specific, 76
sources, 74
storage, 75
template, 77
unit assay, 75
Ribonuclease, 263
RNA polymerases, eukaryotic, 59-72
biological functions, 60
experimental procedure, 64
end labeled RNA, 66
HeLa whole cell lysate, 68
lysolecithin, 69
lysophosphatidylcholine, 69
nuclear extracts, 68
nuclease S1 RNA mapping, 66
primer extension, 65
reverse transcription, 66
run off transcription, 64
S 100 extract, 67
TCA preparation assay, 64
in vitro transcription, 63
ion requirement, 62
properties, 60, 61
RNA transcription, 60
structure, 62
transcription factors, 62
TFIIA, 62, 63
TFIIA, B, 62
TFIIB, 62, 63
TFIID, 62, 63
TFIIE, 62, 63
RNA polymerases I, II, III, 60, see also
RNA polymerases

RNase, 263, see also Ribonuclease

RNase A, 263-270, see also Ribonuclease

RNase H, 273-276

RNase I, 263, see also Ribonuclease

RsaI, 155, see also Restriction enzymes

RspXI, 155, see also Restriction enzymes

RsrII, 155, see also Restriction enzymes

S

S-adenosylmethionine, 108, 202, 203, 204

SAM, see S-adenosylmethionine

SacI, 155, see also Restriction enzymes

Sacll, 155, see also Restriction enzymes

SalI, 156, see also Restriction enzymes

Sau3AI, 156, see also Restriction

enzymes

Sau96I, 156, see also Restriction

enzymes

SauI, 156, see also Restriction enzymes

ScaI, 157, see also Restriction enzymes

SctNI, 157, see also Restriction enzymes

ScrFI, 157, see also Restriction enzymes

SduI, 157, see also Restriction enzymes

SdyI, 157, see also Restriction enzymes

SecI, 157, see also Restriction enzymes

SexI, 157, see also Restriction enzymes

SfaNI, 157, see also Restriction enzymes

SfiI, 157, see also Restriction enzymes

SinI, 157, see also Restriction enzymes

SmaI, 157, see also Restriction enzymes

SnaBI, 158, see also Restriction enzymes

SnoI, 158, see also Restriction enzymes

Specificity, see each enzyme

SpeI, 158, see also Restriction enzymes

SphI, 158, see also Restriction enzymes

SplI, 158, see also Restriction enzymes

SpoI, 159, see also Restriction enzymes

SspI, 159, see also Restriction enzymes

SstI, 159, see also Restriction enzymes

SstlI, 159, see also Restriction enzymes

Stability, see Enzyme

"Star" activity, see Restriction enzymes

Storage, see each enzyme

StuI, 159, see also Restriction enzymes

StyI, 159, see also Restriction enzymes