Dairy Chemistry And Biochemistry

MILK PROTEINS 225

Whey

I

Protein

I0^0

pH < 4.6 pH > 4.6

Lactose

Minerals

Cationic exchangeresin

(vistec, Spherosil S) Anion [Spherosil exchangeMn OMA)

\

Eluate \ Retentate / Eluate

minerals

Minerals

t

WPI

Figure 4.41 Production of whey protein isolate (WPI) by ion exchange adsorption (from
Mulvihill, 1992).

trodialysis to less than about 0.02% ash, 8-1s precipitates and can be
removed by centrifugation with a yield of more than 90%.
The ion exchangers used to recover total whey protein (WPI) may also
be used to fractionate whey proteins. All the whey proteins are adsorbed
initially on Spherosil QMA resin but on continued passage of whey through